Pharmacokinetics and ex vivo pharmacodynamics of Minocycline against Salmonella abortus equi in donkey plasma and tissue cage fluid
| Autor: | Wenqiang Liu, Xiuling Chu, Zaijian Li, Jianqing Su, Changfa Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Salmonella 040301 veterinary sciences Cmax Minocycline Absorption (skin) Microbial Sensitivity Tests Pharmacology medicine.disease_cause Injections Intramuscular 0403 veterinary science 03 medical and health sciences Pharmacokinetics medicine Animals 030304 developmental biology 0303 health sciences General Veterinary Chemistry 04 agricultural and veterinary sciences Equidae Anti-Bacterial Agents Pharmacodynamics Area Under Curve Diffusion Chambers Culture Female Donkey Ex vivo medicine.drug |
| Zdroj: | Research in veterinary science. 135 |
| ISSN: | 1532-2661 |
| Popis: | Tissue Cage (TC) model was used to evaluate the pharmacokinetics and ex vivo pharmacodynamics of Minocycline (MINO) after intramuscular (IM) administration to donkeys at 4 mg/kg body-weight. The Cmax of MINO with 1.79 and 2.63 μg mL−1 was obtained at 2.96 and 1.41 h in TCF (tissue cage fluid) and plasma respectively. The absorption half-lives (t1/2ka) of MINO were calculated to be 0.71 h in TCF and 0.32 h in plasma, whereas the elimination half-lives (t1/2ke) were 10.46 h in TCF and 5.95 h in plasma. The distribution volume (Vd/F) of MINO was estimated to be 1.84 L kg−1 in TCF and 1.28 L kg−1 in plasma. The total clearance (CLb/F) of MINO was computed as 0.12 and 0.15 L/ (h·kg) in TCF and plasma respectively. The area under the concentration-time curve (AUC) of MINO was 32.77 μg mL−1h in TCF and 25.27 μg mL−1h in plasma, respectively.The ex vivo time-kill curves were established for plasma and TCF samples using Salmonella abortus equi. The MIC and MBC of MINO against salmonella were 0.08 and 0.16 μg mL−1 for plasma, 0.04 and 0.08 μg mL−1 for TCF. The plasma Cmax/MIC and AUC/MIC values after IM administration were 32.88 ± 9.87 and 315.88 ± 42.65 h, respectively. The TCF Cmax/MIC and AUC/MIC values after IM administration were 44.75 ± 9.32 and 819.25 ± 65.23 h, respectively. The values of T > MIC were approximately >36 h in plasma and > 65 h in TCF. These findings from this study suggest that MINO may be therapeutically effective in diseases of donkeys caused by salmonella when used at a dose of 4 mg/kg IM administration. |
| Databáze: | OpenAIRE |
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