Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii

Autor: Denise Vizziano-Cantonnet, Gabriela Bedó, Frédéric Brunet, Julio Berbejillo, Jean-Nicolas Volff, Anabel Martinez-Bengochea
Přispěvatelé: Universidad de la República, Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Programa de Desarrollo Tecnologico del Uruguay [71-13], Universidad de la República [Montevideo] (UDELAR), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Sex Differentiation
DETERMINING REGION
[SDV]Life Sciences [q-bio]
TILAPIA OREOCHROMIS-NILOTICUS
I MESSENGER-RNA
Sturgeon
Testis
DM-DOMAIN GENE
Insulin-Like Growth Factor I
Phylogeny
SEQUENCE ALIGNMENT
0303 health sciences
Fishes
Steroid 17-alpha-Hydroxylase
Cell Differentiation
04 agricultural and veterinary sciences
Acipenser baerii
Sertoli cell
medicine.anatomical_structure
Development of the gonads
Fish Proteins
medicine.medical_specialty
endocrine system
medicine.drug_class
RAINBOW-TROUT
SOX9
Biology
Andrology
03 medical and health sciences
Internal medicine
Genetics
medicine
Animals
OVARY DEVELOPMENT
[INFO]Computer Science [cs]
14. Life underwater
030304 developmental biology
GONADAL DIFFERENTIATION
Sexual differentiation
TELEOST FISH
Cell Biology
Phosphoproteins
biology.organism_classification
Androgen
ONCORHYNCHUS-MYKISS
Sexual dimorphism
Endocrinology
Gene Expression Regulation
040102 fisheries
0401 agriculture
forestry
and fisheries
Transcriptome
Transcription Factors
Developmental Biology
Zdroj: Molecular Reproduction and Development
Molecular Reproduction and Development, Wiley, 2012, 79 (8), pp.504-516. ⟨10.1002/mrd.22053⟩
Molecular Reproduction and Development, 2012, 79 (8), pp.504-516. ⟨10.1002/mrd.22053⟩
ISSN: 1040-452X
1098-2795
DOI: 10.1002/mrd.22053⟩
Popis: International audience; The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii, by expression profiling of Sertoli cell transcription factors (dmrt1, sox9) that control testis differentiation in vertebrates; Leydig cell factors (cyp17a1, star) affecting androgen production; the androgen receptor (ar); a growth factor controlling testis development (igf1); and a gene coding for a gonadotropin hormone (lh). Two genes were characterised for the first time in the Siberian sturgeon (dmrt1, cyp17a1), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex-dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre- and post-differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post-differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9. The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors (dmrt1, sox9) and of genes involved in the production and receptivity of androgens (cyp17a1, star and ar) together with lh. Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.Mol. Reprod. Dev. 79: 504-504, 2012. (C) 2012 Wiley Periodicals, Inc.
Databáze: OpenAIRE
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