Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?
| Autor: | Mohammad Reza Afrooz, Mohammad Kazemi Arababadi, Vahid Mirzaei, Hamid Daneshvar, Reza Bidaki, Ziba Shabani, Masoud Karimi-Googheri, Maryam Ebrahim, Yaser Yousefpoor, Mehdi Khaleghinia |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Hepatitis B virus Interferon-Induced Helicase IFIH1 Hepatitis B virus DNA polymerase viruses Immunology Gene Expression Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Hepatitis B virus PRE beta DEAD-box RNA Helicases Hepatitis B Chronic Immune system Virology Gene expression medicine Humans Hepatitis B e Antigens RNA Messenger Receptors Immunologic Gene Expression Profiling virus diseases Viral Load Hepatitis B medicine.disease Cross-Sectional Studies Real-time polymerase chain reaction DEAD Box Protein 58 Molecular Medicine Female Viral load |
| Zdroj: | Viral Immunology. 28:504-508 |
| ISSN: | 1557-8976 0882-8245 |
| DOI: | 10.1089/vim.2015.0056 |
| Popis: | Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of the present study was to evaluate the mRNA levels of MDA5 and RIG-1 in the peripheral blood immune cells of CHB patients in comparison to healthy controls. In this cross-sectional study, the mRNA levels of MDA5 and RIG-1 were examined in 60 CHB patients and 60 healthy controls using the real-time polymerase chain reaction (PCR) technique. Our results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in CHB patients when compared to healthy controls. Our results also revealed that mRNA levels of MDA5 and RIG-1 were not altered among CHB patients with various states of e-antigen of hepatitis B and HBV-DNA viral loads. According to the results presented here, it may be concluded that downregulation of MDA5 may be a responsible mechanism from several reasons, which leads to HBV persistence in CHB patients. |
| Databáze: | OpenAIRE |
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