Low Burst Promoting Activity Production by Phytohemagglutinin-Stimulated Mononuclear Cells of Cord Blood
| Autor: | Takeshi Miyanomae, Yoshitaka Iwai, Mayumi Tanaka, Haruki Mikawa, Mitsufumi Mayumi, Tomohide Yoshimura |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
chemical and pharmacologic phenomena
Stimulation Umbilical cord Peripheral blood mononuclear cell Monocytes Andrology Colony-Stimulating Factors Humans Medicine Erythropoiesis Phytohemagglutinins Incubation Cells Cultured Dose-Response Relationship Drug business.industry Cell growth Infant Newborn Fetal Blood Hematopoietic Stem Cells Stimulation Chemical Culture Media Electrophysiology Dose–response relationship medicine.anatomical_structure Cord blood Pediatrics Perinatology and Child Health Immunology business Thymidine |
| Zdroj: | Pediatric Research. 21:285-288 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-198703000-00017 |
| Popis: | Burst-promoting activity (BPA) produced by phytohemagglutinin(PHA)-stimulated cord blood mononuclear cells (MNC) was examined using the two-stage cell culture assays. Burst-promoting activity was measured as the increase in the number of early erythroid progenitor cells in 2-day incubation of peripheral blood MNC with or without the conditioned medium of PHA-stimulated MNC (PHA-LCM). Burst-promoting activity in PHA-LCM of cord blood was significantly lower than that of adult blood (37 +/- 13 versus 105 +/- 19%, mean +/- SD, p less than 0.01). No elevation of inhibitors to the erythroid colony growth was noted in PHA-LCM of cord blood. In contrary, the response of cord blood MNC to PHA was similar to that of adult blood MNC, as determined by colony-stimulating activity production and cell proliferation. These results showed that burst-promoting activity production by PHA-stimulated MNC of cord blood was lower than that of adult blood. |
| Databáze: | OpenAIRE |
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