Mechanism of Radiosensitization by Porphyrins
| Autor: | Zivile Luksiene, Danute Labeikyte, Benediktas Juodka, Johan Emelian Moan |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Radiation-Sensitizing Agents
Health Toxicology and Mutagenesis medicine.medical_treatment Mitosis Apoptosis Photodynamic therapy Toxicology Antioxidants Pathology and Forensic Medicine Mice chemistry.chemical_compound Radiation Ionizing NAD(P)H Dehydrogenase (Quinone) medicine Animals Photosensitizer Carcinoma Ehrlich Tumor Receptor Glutathione Transferase Hematoporphyrin Photosensitizing Agents Superoxide Dismutase Chemistry Cell Cycle DNA General Medicine Catalase Receptors GABA-A Ligand (biochemistry) Hematoporphyrins Glutathione Reductase Biochemistry Cancer research Growth inhibition |
| Zdroj: | Journal of Environmental Pathology, Toxicology and Oncology. 25:293-306 |
| ISSN: | 0731-8898 |
| DOI: | 10.1615/jenvironpatholtoxicoloncol.v25.i1-2.190 |
| Popis: | According to our previous data, hematoporphyrin dimethyl ether (HPde) at concentrations useful for photodynamic therapy can radiosensitize aggressive Ehrlich ascite carcinoma (EAT) to 2Gy irradiation inducing total tumour growth inhibition. The aim of this study was to further investigate the possible mechanism of radiosensitization of EAT by dicarboxylic porphyrin-HPde. Our results reveal that HPde is inducing several rearrangements in the EAT cells: 1.2 x 10 -6 M of the photosensitizer diminishes the number of cells in mitosis by a factor of 3, increases the number of cells in the S phase of the cell cycle, modifies the activities of antioxidant enzymes glutation S-transferase (GST) and DT-diaphorase (DTD), and eventually induces slight apoptosis. Moreover, it was shown that HPde is a ligand of peripheral benzodiazepine receptor (PBR). Named "house keeper," PBR is usually responsible for all these perturbations, which, in our case, act in concert with the following ionizing radiation, producing the interaction of two antiproliferative/destructive factors. |
| Databáze: | OpenAIRE |
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