Dual-Specific Interaction to Detect DNA on Gold Nanoparticles
| Autor: | Di Zhang, Jiajun Gu, Qinglei Liu, Shenmin Zhu, Xiaoqiu Dou, Andrew Tobias Aveling Jenkins, Chuanliang Feng, Wang Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Streptavidin
Fluorophore DNA hybridization quenching Nanoparticle Metal Nanoparticles Nanotechnology lcsh:Chemical technology sensors Biochemistry Article Analytical Chemistry chemistry.chemical_compound Biotin lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation chemistry.chemical_classification DNA–DNA hybridization Biomolecule technology industry and agriculture DNA Carbocyanines streptavidin/biotin Atomic and Molecular Physics and Optics Solutions chemistry Colloidal gold fluorophore dye nanoparticles Spectrophotometry Ultraviolet Gold |
| Zdroj: | Sensors (Basel, Switzerland) Feng, C-L, Dou, X-Q, Liu, Q-L, Zhang, W, Gu, J-J, Zhu, S-M, Jenkins, A T A & Zhang, D 2013, ' Dual-specific interaction to detect DNA on gold nanoparticles ', Sensors, vol. 13, no. 5, pp. 5749-5756 . https://doi.org/10.3390/s130505749 Sensors Volume 13 Issue 5 Pages 5749-5756 Sensors, Vol 13, Iss 5, Pp 5749-5756 (2013) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s130505749 |
| Popis: | An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules. |
| Databáze: | OpenAIRE |
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