Analysis of optically active compounds using conventional chromatography with a circular dichroism detector
| Autor: | L Blok-Tip, P.M.J.M Jongen, D. de Kaste, K D Hartog, C. van Alphen, C. Slijkhuis |
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| Rok vydání: | 2003 |
| Předmět: |
Circular dichroism
Chromatography Optical Rotation Chemistry Circular Dichroism Clinical Biochemistry Analytical chemistry Pharmaceutical Science Reversed-phase chromatography High-performance liquid chromatography Analytical Chemistry Chiral column chromatography Capillary electrophoresis Pharmaceutical Preparations Reagent Drug Discovery Enantiomer Chiral derivatizing agent Chromatography High Pressure Liquid Spectroscopy |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 32:905-912 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(03)00192-4 |
| Popis: | Analysis of optically active compounds in complex samples is often based on chiral chromatography or capillary electrophoresis in order to separate the enantiomers. This requires a chiral reagent, when using conventional chromatography, or an expensive chiral column, or a chiral selector, when using capillary electrophoresis. The type of column, reagent, or additive depends highly on the compound to be analysed. A simple and generally applicable method is using a conventional HPLC column coupled to a CD detector. Separation of enantiomers is not required, as they can be identified by a positive or negative peak. A racemate does not produce a peak; neither does an optically inactive compound. The application of HPLC-CD for the identification of pharmacologically active compounds, such as dexamphetamine, 5-hydroxytryptophan, (-)-huperzine A, and interferon, as standards, in registered drugs, in falsifications, and in food supplements is described. |
| Databáze: | OpenAIRE |
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