Honokiol and magnolol induce Ca2+ mobilization in rat cortical neurons and human neuroblastoma SH-SY5Y cells
| Autor: | Kousuke Nakade, Yasuhide Mitsumoto, Yoshiyasu Fukuyama, Haifeng Zhai |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Honokiol
SH-SY5Y Phospholipase Biology Lignans Rats Sprague-Dawley chemistry.chemical_compound Neuroblastoma Pregnancy Cell Line Tumor Extracellular Animals Humans Cells Cultured Pharmacology Cerebral Cortex Neurons Fluo-3 Phospholipase C Biphenyl Compounds Magnolol Cell biology Rats chemistry Calcium Female Neuroscience Intracellular |
| Zdroj: | European journal of pharmacology. 474(2-3) |
| ISSN: | 0014-2999 |
| Popis: | We examined the intracellular Ca 2+ response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis. In these two kinds of neuronal cells, honokiol and magnolol increased cytoplasmic free Ca 2+ with a characteristic lag phase. The cytoplasmic free Ca 2+ increase was independent of extracellular Ca 2+ , but dependent on activation of phospholipase C and inositol 1,4,5-triphosphate (IP 3 ) receptors. These results suggest that honokiol and magnolol increase cytoplasmic free Ca 2+ through a phospholipase C-mediated pathway, and that the release of Ca 2+ from intracellular stores mainly contributes to the increase in cytoplasmic free Ca 2+ . Thus, honokiol and magnolol may be involved in a new activation mechanism closely associated with intracellular Ca 2+ mobilization. |
| Databáze: | OpenAIRE |
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