Morin Induces Melanogenesis via Activation of MAPK Signaling Pathways in B16F10 Mouse Melanoma Cells
| Autor: | Nuri Song, JaeYeon Ko, Minjeong Kim, SeoYeon Shin, Kyung-Mok Park |
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| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Cell Survival MAP Kinase Signaling System p38 mitogen-activated protein kinases Tyrosinase Melanoma Experimental Pharmaceutical Science p38 Morin tyrosinase Models Biological Article Analytical Chemistry lcsh:QD241-441 Melanin Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Cell Line Tumor Drug Discovery Animals Phosphorylation Physical and Theoretical Chemistry Protein Kinase Inhibitors Transcription factor 030304 developmental biology Flavonoids Melanins MITF 0303 health sciences integumentary system Monophenol Monooxygenase Chemistry Organic Chemistry Microphthalmia-associated transcription factor morin melanin Cell biology ERK Chemistry (miscellaneous) 030220 oncology & carcinogenesis TRP-1/2 Molecular Medicine Agaricales Intracellular |
| Zdroj: | Molecules, Vol 26, Iss 2150, p 2150 (2021) Molecules Volume 26 Issue 8 |
| ISSN: | 1420-3049 |
| Popis: | Morin is a well-known flavonoid, and has been reported to have various properties, such as anti-cell death, antioxidant, and anti-inflammatory properties. Although studies on the biochemical and biological actions of morin have been reported, the melanin biosynthesis effects and molecular mechanisms are unknown. In this study, we first found that morin has the effect of enhancing melanin biosynthesis in B16F10 mouse melanoma cells, and analyzed the molecular mechanism. In this study, we examined the effects of morin on the melanin contents and tyrosinase activity, as well as the protein expression levels of the melanogenic enzymes TRP-1, TRP-2, and microphtalmia-associated transcription factor (MITF) in B16F10 mouse melanoma cells. Morin showed no cytotoxicity in the concentration range of 5–100 μM, and significantly increased the intracellular tyrosinase activity and melanin contents. In mechanism analysis, morin increased the protein expression of TRP-1, TRP-2, and MITF associated with melanogenesis. Furthermore, morin increased phosphorylated ERK and p38 at the early time, and decreased phosphorylated ERK after 12 h. The results suggest that morin enhances melanin synthesis through the MAPK signaling pathways in B16F10 mouse melanoma cells. |
| Databáze: | OpenAIRE |
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