Comparison of Neisseria gonorrhoeae minimum inhibitory concentrations obtained using agar dilution versus microbroth dilution methods
| Autor: | Rachael K. Jacobson, Grant J. Carr, Marysa J. Notaro |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Microbiology (medical)
Fastidious organism Tetracycline medicine.drug_class Antibiotics Colony Count Microbial Microbial Sensitivity Tests Azithromycin medicine.disease_cause Microbiology Agar dilution 03 medical and health sciences Gonorrhea Antibiotic resistance Ciprofloxacin Drug Resistance Bacterial medicine Humans Molecular Biology 030304 developmental biology 0303 health sciences Bacterial disease 030306 microbiology Chemistry Ceftriaxone Neisseria gonorrhoeae Anti-Bacterial Agents Culture Media medicine.drug |
| Zdroj: | Journal of microbiological methods. 157 |
| ISSN: | 1872-8359 |
| Popis: | With increasing antibiotic resistance observed amongst clinical isolates of Neisseria gonorrhoeae , the second most prevalent sexually transmitted bacterial disease in the United States, there is still a need for antimicrobial susceptibility testing (AST). The current method recommended by the Clinical and Laboratory Standards Institute is agar dilution . In this study, we show that a commercially available version of Fastidious Broth is capable of supporting N. gonorrhoeae in the evaluation of minimum inhibitory concentrations of 4 antibiotics (ceftriaxone, azithromycin, ciprofloxacin , and tetracycline), when comparing the agar dilution (AD) versus microbroth dilution (MBD) method and the susceptibilities obtained for 32 N. gonorrhoeae isolates. Herein, 3 out of the 4 antibiotics tested showed 94% or greater essential agreement (EA) and 91% or greater categorical agreement (CA) respectively, when comparing the MBD versus AD methods. |
| Databáze: | OpenAIRE |
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