Optimization of solid-phase clean-up prior to liquid chromatographic analysis of ochratoxin A in roasted coffee
| Autor: | Liberty Sibanda, Carlos Van Peteghem, Sarah De Saeger |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Ochratoxin A
Detection limit Chromatography Organic Chemistry General Medicine Mycotoxins Coffee Ochratoxins Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Spectrometry Fluorescence chemistry Sample preparation Solid phase extraction Methanol Ochratoxin Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 959:327-330 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(02)00408-9 |
| Popis: | An extraction and clean-up method for ochratoxin A (OA) in roasted coffee has been developed and the HPLC method optimized. An interfering compound with a similar retention time as OA was adsorbed by the aminopropyl (NH2) material at < or = 5% NaHCO3. Residual OA on the column was recovered by washing with the extraction solution followed with methanol. Fractions were mixed together for further clean-up with Ochratest immunoaffinity columns (IACs). Analysis by HPLC resulted in a well resolved OA peak and reduction in matrix interferences. Recoveries ranged from 72 to 84% and the detection limit was 1 ng/g. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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