Optical Properties of Methyl Orange-Doped Droplet and Photodynamic Therapy of Staphylococcus aureus
Autor: | Mohammad Reza Sharifmoghadam, Shurshalova Guzel Salavatovna, Soheil Sharifi, Forough Rakhshanizadeh, Afshin Azarpour, Gholamhossein Zohuri |
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Rok vydání: | 2019 |
Předmět: |
endocrine system
Staphylococcus aureus Sociology and Political Science Surface Properties Sodium Clinical Biochemistry chemistry.chemical_element Microbial Sensitivity Tests 010402 general chemistry 01 natural sciences Biochemistry chemistry.chemical_compound Pulmonary surfactant pH indicator Fluorometer Methyl orange Particle Size Spectroscopy Benzene Fluorescent Dyes 010405 organic chemistry Optical Imaging technology industry and agriculture eye diseases 0104 chemical sciences Anti-Bacterial Agents Hexane Clinical Psychology Spectrometry Fluorescence chemistry Photochemotherapy Law Azo Compounds Social Sciences (miscellaneous) Nuclear chemistry |
Zdroj: | Journal of fluorescence. 29(6) |
ISSN: | 1573-4994 |
Popis: | Dye-doped droplets are known as mixtures of dyes with uniform solutions of water droplets in a continuous phase of oils with surfactants. To observe the relationship between water droplet structures and surfactant types on optical properties of dyes, a mixture of methyl orange (MO)-doped droplet prepared with benzane and hexane as oils and sodium bis(2-ethylhexyl) sulfosuccinate (AOT) as a surfactant was thus examined using Z-scan instrument, spectrophotometer, and fluorimeter in the present study. The findings revealed that nonlinear refractive (NLR) index, nonlinear absorption (NLA) coefficient, as well as fluorescence intensity of the MO had enhanced following a reduction in the droplet water content induced by changes in the non-centrosymmetric charge density distribution of this pH indicator. Moreover, the MO-doped droplet in a continuous phase of benzene investigated by 1H nuclear magnetic resonance (NMR) spectroscopy indicated that the MO had been located in the droplet in the vicinity of the hydrophilic part of the surfactant. Furthermore, the MO-doped droplets along with laser radiation were employed to perform antibacterial photodynamic therapy (APDT) of Staphylococcus aureus (S. aureus). It was ultimately concluded that the bacteria colony had also extremely diminished in the group treated by the MO-doped droplet. |
Databáze: | OpenAIRE |
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