Effective glycemic control achieved by transplanting non-viral cationic liposome-mediated VEGF-transfected islets in streptozotocin-induced diabetic mice
| Autor: | You Ran Ahn, Moon Kyu Lee, Seung Hoon Oh, Jae Hoon Chung, Myung-Shik Lee, Hee Young Chae, Kwang Won Kim, Yong Ki Min, Byung Wan Lee |
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| Rok vydání: | 2006 |
| Předmět: |
Male
endocrine system medicine.medical_specialty endocrine system diseases Cell Survival Genetic enhancement Clinical Biochemistry Islets of Langerhans Transplantation Neovascularization Physiologic Biology Transfection Biochemistry Streptozocin Diabetes Mellitus Experimental chemistry.chemical_compound Islets of Langerhans Mice Internal medicine Diabetes mellitus Insulin Secretion medicine Animals Insulin Cationic liposome RNA Messenger Molecular Biology geography Mice Inbred BALB C geography.geographical_feature_category Vascular Endothelial Growth Factors Body Weight Glucose Tolerance Test Streptozotocin medicine.disease Islet Transplantation Vascular endothelial growth factor Disease Models Animal Endocrinology Glucose chemistry Hyperglycemia Liposomes Molecular Medicine medicine.drug |
| Zdroj: | Experimentalmolecular medicine. 37(6) |
| ISSN: | 1226-3613 |
| Popis: | Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 ㎍/μL cDNA and 25 μL effectene and cell viabilities were also satisfactory under this condition, and the overproduction of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (nondiabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable islets and increased VEGF-transfected grafts vascularization were observed in renal capsules. These studies support the usefulness of VEGFtransfected islet delivery using a cationic lipid reagent to achieve euglycemia using a minimal number of islets. |
| Databáze: | OpenAIRE |
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