Adjuvant-increased glyphosate uptake by protoplasts isolated from Quack Grass Elytrigia repens (L.) Nevski
| Autor: | H. de Ruiter, E. Meinen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
0106 biological sciences
Ammonium sulfate medicine.medical_treatment 2-aminoisobutyric acid Plant Science 01 natural sciences chemistry.chemical_compound Pulmonary surfactant Botany Surfactant medicine Incubation Research Institute for Agrobiology and Soil Fertility Instituut voor Agrobiologisch en Bodemvruchtbaarheidsonderzoek 04 agricultural and veterinary sciences Protoplast 2-Aminoisobutyric acid Staining 010602 entomology chemistry Glyphosate 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Cell membrane Agronomy and Crop Science Adjuvant Nuclear chemistry |
| Zdroj: | Weed Science 44 (1996) 1 |
| ISSN: | 0043-1745 |
| DOI: | 10.1017/s0043174500093528 |
| Popis: | Protoplasts were isolated from quackgrass to investigate the influence of two surfactants and ammonium sulfate on glyphosate uptake. Without adjuvants, the uptake of glyphosate was not detectable. The uptake of the reference compound 2-aminoisobutyric acid was linear with time and was inhibited 60% in the presence of 10 μM carbonyl cyanidem-chlorophenylhydrazone. Glyphosate uptake increased in the presence of both surfactants and ammonium sulfate. After 0.5 h of incubation the internal glyphosate concentration was 37% of the external concentration (100 μM) in the presence of 0.025 g L−1Ethomeen T/25, 21% in the presence of 0.25 g L−1Atplus 201, 13% with 5 mM ammonium sulfate, and 20% with 50 mM ammonium sulfate. The surfactant treatments did not reduce either the viability or the internal pH (= 7.1) as judged by fluorescein diacetate staining or 5,5-dimethyloxazolidine-2,4-dione distribution, respectively. The treatments with ammonium sulfate slightly increased the internal pH. The concentration of Ethomeen T/25 required to disrupt the protoplasts was 0.075 g L−1; Atplus 201 did not disrupt the protoplasts at concentrations up to 10 g L−1. |
| Databáze: | OpenAIRE |
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