Isolation of canine Anaplasma phagocytophilum strains from clinical blood samples using the Ixodes ricinus cell line IRE/CTVM20
| Autor: | Nikola Pantchev, Lesley Bell-Sakyi, Christine Geiger, Inke Krupka, Viktor Dyachenko, Reinhard K. Straubinger, M. Majzoub |
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| Jazyk: | angličtina |
| Předmět: |
Male
Anaplasmosis animal diseases CATTLE IRE/CTVM20 HORSES HUMAN GRANULOCYTIC EHRLICHIOSIS CULTURE 0302 clinical medicine RNA Ribosomal 16S INFECTION Dog Dog Diseases TICK AGENT Infectivity 0303 health sciences biology MARGINALE RICKETTSIALES General Medicine 3. Good health Ixodes scapularis Ehrlichiosis (canine) Female EQUI Anaplasma phagocytophilum Ixodes ricinus Short Communication 030231 tropical medicine HL-60 Cells Tick Microbiology Cell Line 03 medical and health sciences Dogs parasitic diseases medicine Electron microscopy Animals Humans General Veterinary Ixodes 030306 microbiology fungi Ehrlichiosis biology.organism_classification medicine.disease bacterial infections and mycoses Virology veterinary(all) CULTIVATION Tick cell lines |
| Zdroj: | Veterinary Microbiology Dyachenko, V, Geiger, C, Pantchev, N, Majzoub, M, Sakyi, L, Krupka, I & Straubinger, R K 2013, ' Isolation of canine Anaplasma phagocytophilum strains from clinical blood samples using the Ixodes ricinus cell line IRE/CTVM20 ', Veterinary Microbiology, vol. 162, no. 2-4, pp. 980-986 . https://doi.org/10.1016/j.vetmic.2012.10.021 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2012.10.021 |
| Popis: | Anaplasma phagocytophilum is an intracellular tick-borne rickettsial pathogen, which causes granulocytic anaplasmosis in various species of livestock and companion animals and also in humans. Previously A. phagocytophilum has been isolated and propagated in cell lines derived from the tick Ixodes scapularis and in the human promyelocytic cell line HL60. In this study we used the Ixodes ricinus-derived cell line IRE/CTVM20 to isolate and propagate two new canine strains of A. phagocytophilum.Blood samples were collected by veterinarians from two dogs, one from Germany and the other from Austria. Suspicion of clinical canine granulocytic anaplasmosis was raised by the treating veterinarians and after confirmation of A. phagocytophilum infection by real-time PCR, buffy coat cells were isolated and co-cultivated with IRE/CTVM20 cells maintained at 28 degrees C in L15/L15B medium.In the tick cells, rickettsial inclusions were first recognised after 86 days of incubation. Electron microscopic examination of tick cells infected with one of the isolates revealed cytoplasmic vacuoles containing pleomorphic organisms with individual bacteria enveloped by a bilayer membrane. Sequencing of 16S rRNA genes confirmed the isolation of A. phagocytophilum and showed the highest identity to the A. phagocytophilum human HZ strain. The two A. phagocytophilum isolates were passaged several times in IRE/CTVM20 cells and transferred to the L scapularis cell line ISE6. This confirms for the first time the successful establishment and continuous cultivation of this pathogen in I. ricinus cells as well as infectivity of these canine strains for I. scapularis cells. (C) 2012 Published by Elsevier B.V. |
| Databáze: | OpenAIRE |
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