Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings
| Autor: | Xiao Meng Zhang, Wei Jie Jiang, Wen Chao Zhao, Xue Zhang, Xue Yong Yang, Qiang Li, Hongjun Yu |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Oxygenase Physiology Nitrogen Glutathione reductase Plant Science Ascorbic Acid Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene Expression Regulation Plant Genetics chemistry.chemical_classification Nitrogen deficiency APX Ascorbic acid Enzyme assay Plant Leaves 030104 developmental biology Enzyme Glutathione Reductase chemistry Biochemistry Seedlings biology.protein Ascorbate Oxidase Cucumis sativus 010606 plant biology & botany |
| Zdroj: | Plant physiology and biochemistry : PPB. 108 |
| ISSN: | 1873-2690 |
| Popis: | L-Ascorbic acid (AsA, ascorbate) is one of the most abundant natural antioxidants, and it is an important factor in the nutritional quality of cucumber. In this work, key enzymes involved in the ascorbic acid biosynthesis and recycling pathway in cucumber seedlings under nitrogen deficiency were investigated at the levels of transcription and enzyme activity. The activities of myo-inositol oxygenase (MIOX) and transcript levels of MIOXs increased dramatically, while the activities of ascorbate oxidase (AO) and glutathione reductase (GR) and transcript levels of AOs and GR2 decreased significantly in N-limited leaves, as did the ascorbate concentration, in nitrogen-deficient cucumber seedlings. The activities of other enzymes and transcript levels of other genes involved in the ascorbate recycling pathway and ascorbate synthesis pathways decreased or remained unchanged under nitrogen deficiency. These results indicate that nitrogen deficiency induced genes involved in the ascorbate-glutathione recycling and myo-inositol pathway in cucumber leaves. Thus, the AO, GR and MIOX involved in the pathways might play roles in AsA accumulation. |
| Databáze: | OpenAIRE |
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