Tetracycline-loaded calcium phosphate nanoparticle (Tet-CPNP): Rejuvenation of an obsolete antibiotic to further action
| Autor: | Debanjan Dutta, Milon Banik, Tarakdas Basu, Mousumi Patra, Riya Mukherjee |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Calcium Phosphates
0301 basic medicine food.ingredient Tetracycline medicine.drug_class 030106 microbiology Antibiotics Biophysics 02 engineering and technology medicine.disease_cause Biochemistry Microbiology 03 medical and health sciences Minimum inhibitory concentration food Shigella flexneri Drug Resistance Multiple Bacterial medicine Animals Rejuvenation Agar Shigella Particle Size Molecular Biology Zebrafish Protein Synthesis Inhibitors Bacteria biology Cell Membrane 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents Cell killing Larva Nanoparticles 0210 nano-technology medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1860:1929-1941 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2016.06.006 |
| Popis: | Background Increasing resistance in bacteria towards antibiotics has made it imperative to research on their revitalization to combat infectious diseases. This study dealt with synthesis of a nano-form of the antibiotic tetracycline, its characterization and potency of killing different multi-drug resistant diarrhea-causing bacteria. Methods Nano-formulation was done by loading tetracycline within biocompatible calcium phosphate nanoparticle. The synthesized tetracycline-loaded calcium phosphate nanoparticle (Tet-CPNP) was characterized by the techniques like TEM, DLS, EDS, FTIR, spectrofluorimetry and dialysis. Bactericidal activity of nano-particulate tetracycline was investigated by agar plating, spectrophotometry, phase contrast-fluorescence-atomic force microscopy and flow cytometry techniques. Results The Tet-CPNPs were 8 ± 5 nm in size and nearly spherical in shape, efficiency of tetracycline loading in CPNP was about 20% and the release of antibiotic from Tet-CPNPs was sustainable during 7 days. Minimum inhibitory concentration (MIC) of Tet-CPNP on multiple antibiotic (including tetracycline) resistant bacteria like Escherichia coli , Salmonella kentuckey and Shigella flexneri was in the range of 20–40 μg/ml, whereas MIC of free tetracycline was in the range of 150–180 μg/ml. NP-mediated cell filamentation and cell membrane disintegration caused cell killing. Moreover, death of Shigella -infected Zebra fish larvae was stalled by Tet-CPNP treatment. CPNP itself had no toxic effect on bacteria as well as on Zebra fish. Conclusion Our nano-formulation of tetracycline might reclaim a nearly obsolete antibiotic to further potential function. General significance Such a study on revival of an old, cheap, broad-spectrum antibiotic to further action is highly beneficial to developing countries with limited health care budgets. |
| Databáze: | OpenAIRE |
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