In vivo effects of olive oil and trans-fatty acids on miR-134, miR-132, miR-124-1, miR-9-3 and mTORC1 gene expression in a DMBA-treated mouse model
| Autor: | Richard Molnar, István Kiss, Nowrasteh Ghodratollah, László Szabó, Richard Darago, Tímea Varjas, Balázs Németh, Arpad Deutsch, Andras Tomesz, Ferenc Budán |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Physiology
DMBA Gene Expression mTORC1 Mice Plant Products Immune Physiology Gene expression Medicine and Health Sciences Kidney Multidisciplinary Chemistry Liver Diseases Eukaryota Olives Agriculture Trans Fatty Acids Plants medicine.anatomical_structure Oncology Nephrology Renal Cancer Medicine Female Anatomy Research Article medicine.medical_specialty Science 9 10-Dimethyl-1 2-benzanthracene Spleen Gastroenterology and Hepatology Mechanistic Target of Rapamycin Complex 1 Vegetable Oils Fruits In vivo Internal medicine Gastrointestinal Tumors medicine Genetics Animals Olive Oil PI3K/AKT/mTOR pathway Carcinogen Carcinoma Organisms Renal Cell Carcinoma Biology and Life Sciences Cancers and Neoplasms Kidneys Renal System Hepatocellular Carcinoma Agronomy MicroRNAs Genitourinary Tract Tumors Endocrinology Gene Expression Regulation Crop Science |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0246022 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Both the intake of beneficial olive oil and of harmful trans-fatty acids (TFAs) in consumed foods are of great significance in tumor biology. In our present study we examined the effects they exert on the expression patterns of miR-134, miR-132, miR-124-1, miR-9-3 and mTOR in the liver, spleen and kidney of mice treated with 7,12-dimethylbenz [a] anthracene (DMBA). Feeding of TFA-containing diet significantly increased the expression of all studied miRs and mTORC1 in all organs examined, except the expression of mTORC1 in the spleen and kidney. Diet containing olive oil significantly reduced the expression of miR-124-1, miR-9-3 and mTORC1 in the liver and spleen. In the kidney, apart from the mTORC1 gene, the expression of all miRs examined significantly decreased compared to the DMBA control. According to our results, the cell membrane protective, antioxidant, and anti-inflammatory effects of olive oil and the cell membrane damaging, inflammatory, and carcinogenic properties of TFA suggest negative feedback regulatory mechanisms. In contrast to our expectations, mTORC1 gene expression in the kidney has not been shown to be an appropriate biomarker–presumably, because the many complex effects that regulate mTOR expression may quench each other. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|