Antineuronal antibodies in idiopathic achalasia and gastro-oesophageal reflux disease
| Autor: | R I Rothstein, J B Meddings, M R Anees, Gary M. Mawe, W Ho, L M Ellis, Peter L. Moses, Keith A. Sharkey |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Pathology Blotting Western Guinea Pigs Fluorescent Antibody Technique Myenteric Plexus Achalasia Ileum digestive system Gastroenterology Antibodies Mice Esophagus Species Specificity Intestinal mucosa Internal medicine otorhinolaryngologic diseases medicine Animals Humans Intestinal Mucosa Myenteric plexus Cell Nucleus Neurons Esophageal disease business.industry digestive oral and skin physiology Proteins medicine.disease digestive system diseases Esophageal Achalasia Oesophagus medicine.anatomical_structure nervous system Gastroesophageal Reflux Immunohistochemistry business Immunostaining |
| Zdroj: | Gut. 52:629-636 |
| ISSN: | 0017-5749 |
| Popis: | Background and aims: The precise aetiology of achalasia is unknown although autoimmunity has been implicated and is supported by several studies. We screened sera from patients with achalasia or gastro-oesophageal reflux disease (GORD) to test for circulating antimyenteric neuronal antibodies. Methods: Serum was obtained from 45 individuals with achalasia, 16 with GORD, and 22 normal controls. Serum was used in immunohistochemistry to label whole mount preparations of ileum and oesophagus of the guinea pig and mouse. Also, sections of superior cervical and dorsal root ganglia, and spinal cord were examined. Results: Positive immunostaining of the myenteric plexus was detected in significantly more achalasia and GORD samples than control samples (achalasia, p |
| Databáze: | OpenAIRE |
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