Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents Staphylococcus aureus from Adhering to Device Surfaces

Autor:	Hang Yang, Jin He, Dehua Luo, Wan Yang, Vijay Singh Gondil, Hongping Wei
Rok vydání:	2021
Předmět:	Methicillin-Resistant Staphylococcus aureus Staphylococcus aureus QH301-705.5 antimicrobial agents immobilization Lysin antimicrobial agents Peptide Microbial Sensitivity Tests medicine.disease_cause Article biofilm Bacterial Adhesion Catalysis Microbiology Inorganic Chemistry chemistry.chemical_compound Silicone medicine Humans lysin Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy surface functionalization Cell Proliferation Thermostability chemistry.chemical_classification Organic Chemistry Biofilm General Medicine Staphylococcal Infections Silicon Dioxide Antimicrobial Anti-Bacterial Agents Computer Science Applications Chemistry chemistry Biofilms Catheter-Related Infections silica-binding peptide Surface modification Peptides
Zdroj:	International Journal of Molecular Sciences; Volume 22; Issue 22; Pages: 12544 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 12544, p 12544 (2021)
ISSN:	1422-0067
DOI:	10.3390/ijms222212544
Popis:	Staphylococcal-associated device-related infections (DRIs) represent a significant clinical challenge causing major medical and economic sequelae. Bacterial colonization, proliferation, and biofilm formation after adherence to surfaces of the indwelling device are probably the primary cause of DRIs. To address this issue, we incorporated constructs of silica-binding peptide (SiBP) with ClyF, an anti-staphylococcal lysin, into functionalized coatings to impart bactericidal activity against planktonic and sessile Staphylococcus aureus. An optimized construct, SiBP1-ClyF, exhibited improved thermostability and staphylolytic activity compared to its parental lysin ClyF. SiBP1-ClyF-functionalized coatings were efficient in killing MRSA strain N315 (>99.999% within 1 h) and preventing the growth of static and dynamic S. aureus biofilms on various surfaces, including siliconized glass, silicone-coated latex catheter, and silicone catheter. Additionally, SiBP1-ClyF-immobilized surfaces supported normal attachment and growth of mammalian cells. Although the recycling potential and long-term stability of lysin-immobilized surfaces are still affected by the fragility of biological protein molecules, the present study provides a generic strategy for efficient delivery of bactericidal lysin to solid surfaces, which serves as a new approach to prevent the growth of antibiotic-resistant microorganisms on surfaces in hospital settings and could be adapted for other target pathogens as well.
Databáze:	OpenAIRE
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