Role of genetic background in P selectin-dependent immune surveillance of the central nervous system
| Autor: | Charles A. Janeway, Christophe Viret, Michael D. Carrithers, Irene Visintin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Adoptive cell transfer Genotype Immunology Central nervous system Inflammation CD8-Positive T-Lymphocytes Biology Mice Meninges Th2 Cells medicine Splenocyte Animals Immunology and Allergy Immunologic Surveillance Mice Knockout Mice Inbred BALB C ICAM-1 Th1 Cells Intercellular Adhesion Molecule-1 Epithelium Mice Inbred C57BL Chemotaxis Leukocyte P-Selectin Phenotype medicine.anatomical_structure Neurology Choroid Plexus Tissue Transplantation Encephalitis Female Choroid plexus Neurology (clinical) medicine.symptom Spleen CD8 |
| Zdroj: | Journal of Neuroimmunology. 129:51-57 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/s0165-5728(02)00172-8 |
| Popis: | Although the blood–brain barrier and blood cerebrospinal fluid barrier maintain the central nervous system (CNS) as an immunologically privileged site, T lymphocytes can migrate through unstimulated brain endothelium and epithelium to perform immune surveillance or initiate inflammation. Our prior results suggested that early CNS migration of a CD4 Th1 cell line was facilitated by P selectin (CD62P) in (PL/J×SJL/J)F1 mice. Here, quantitative analysis of migration 2 h following adoptive transfer of fluorescently labeled cells revealed a 53–72% decrease in activated splenocyte, CD4 Th1 and CD8 migration, but not CD4 Th2, in CD62P-deficient C57BL6/J mice. Immunohistochemistry revealed constitutive expression of CD62P within the meninges and choroid plexus epithelia in C57BL6/J and SJL/J, but not BALB/cJ, mice. Activated splenocyte migration was approximately three- to four-fold greater in SJL/J as compared to BALB/cJ mice. Anti-CD62P treatment normalized this difference. Based on these results, we hypothesize that genetically determined kinetics of immune surveillance may regulate the phenotype of subsequent CNS inflammation. |
| Databáze: | OpenAIRE |
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