How to integrate a micropipette into a closed microfluidic system : absorption spectra of an optically trapped erythrocyte
| Autor: | Kerstin Ramser, Ahmed Alrifaiy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chemical content
Materials science ocis:(300.1030) Absorption Absorption spectroscopy Spectrometer Microfluidics Pipette Nanotechnology ocis:(350.4855) Optical tweezers or optical manipulation Atomic and Molecular Physics and Optics ocis:(110.0180) Microscopy Optical tweezers Microscopy Other Medical Engineering ocis:(220.4000) Microstructure fabrication ocis:(170.3880) Medical and biological imaging Annan medicinteknik Absorption (electromagnetic radiation) ocis:(280.2490) Flow diagnostics Biotechnology |
| Zdroj: | Biomedical Optics Express |
| Popis: | In July 2011 a new concept of a closed microfluidic system equipped with a fixed micropipette, optical tweezers and a UV-Vis spectrometer was presented [Biomed. Opt. Express 2, 2299 (2011)]. Figure 1 showed falsely oriented mirrors. To clarify the design of the setup, this erratum presents a correct schematic We present a new concept of integrating a micropipette within a closed microfluidic system equipped with optical tweezers and a UV-Vis spectrometer. A single red blood cell (RBC) was optically trapped and steered in three dimensions towards a micropipette that was integrated in the microfluidic system. Different oxygenation states of the RBC, triggered by altering the oxygen content in the microchannels through a pump system, were optically monitored by a UV-Vis spectrometer. The built setup is aimed to act as a multifunctional system where the biochemical content and the electrophysiological reaction of a single cell can be monitored simultaneously. The system can be used for other applications like single cell sorting, in vitro fertilization or electrophysiological experiments with precise environmental control of the gas-, and chemical content. Validerad; 2011; 20110721 (ahmahm) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|