Characterization of the expression, localization, and secretion of PANDER in α-cells
| Autor: | Bryan A. Wolf, Jason R. Carnegie, Jianmei Wu, Brant R. Burkhardt, Claudia E. Robert-Cooperman, Robert A. Young |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Cell Culture Techniques Gene Expression Biology Arginine Biochemistry Glucagon Article Mice Paracrine signalling Endocrinology Internal medicine Paracrine Communication medicine Animals Insulin Glucose homeostasis Tissue Distribution Secretion Molecular Biology Protein kinase B Cells Cultured In Situ Hybridization Fluorescence PI3K/AKT/mTOR pathway Dose-Response Relationship Drug Mice Inbred C57BL Insulin receptor Glucose Glucagon-Secreting Cells biology.protein Cytokines |
| Zdroj: | Molecular and Cellular Endocrinology. 325:36-45 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2010.05.008 |
| Popis: | The novel islet-specific protein PANcreatic DERived Factor (PANDER; FAM3B) has been extensively characterized with respect to the beta-cell, and these studies suggest a potential function for PANDER in the regulation of glucose homeostasis. Little is known regarding PANDER in pancreatic -cells, which are critically involved in maintaining euglycemia. Here we present the first report elucidating the expression and regulation of PANDER within the alpha-cell. Pander mRNA and protein are detected in alpha-cells, with primary localization to a glucagon-negative granular cytosolic compartment. PANDER secretion from alpha-cells is nutritionally and hormonally regulated by l-arginine and insulin, demonstrating similarities and differences with glucagon. Signaling via the insulin receptor (IR) through the PI3K and Akt/PKB node is required for insulin-stimulated PANDER release. The separate localization of PANDER and glucagon is consistent with their differential regulation, and the effect of insulin suggests a paracrine/endocrine effect on PANDER release. This provides further insight into the potential glucose-regulatory role of PANDER. |
| Databáze: | OpenAIRE |
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