MAFB is dispensable for the fetal testis morphogenesis and the maintenance of spermatogenesis in adult mice

Autor: Hisashi Oishi, Shosei Yoshida, Ahmed A. H. Abdellatif, Toshiaki Usui, Yu Kitadate, Walaa A Basha, Satoru Takahashi, Masafumi Muratani, Risa Okada, Kazunori Hasegawa, Atsuo Ogura, Hany A. El-Shemy, Hossam H. Shawki, Keiji Mochida
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Physiology
Cellular differentiation
Gene Expression
lcsh:Medicine
Biochemistry
Epithelium
Spermatocytes
Animal Cells
Reproductive Physiology
Testis
Medicine and Health Sciences
Testosterone
Testes
Cell Cycle and Cell Division
lcsh:Science
Cells
Cultured
Mice
Knockout
Multidisciplinary
Chromosome Biology
Leydig Cells
Cell Differentiation
Seminiferous Tubules
Sertoli cell
Cell biology
Meiosis
medicine.anatomical_structure
MAFB
Cell Processes
Proto-Oncogene Proteins c-maf
Female
Cellular Types
Anatomy
Genital Anatomy
Germ cell
Research Article
Cell type
endocrine system
MafB Transcription Factor
Biology
03 medical and health sciences
DNA-binding proteins
medicine
Genetics
Animals
Gene Regulation
RNA
Messenger
Transcription Factor MafB
Spermatogenesis
Sertoli Cells
lcsh:R
Reproductive System
Biology and Life Sciences
Proteins
Epithelial Cells
Cell Biology
Embryonic stem cell
Sperm
Spermatogonia
Regulatory Proteins
Mice
Inbred C57BL
030104 developmental biology
Fertility
Biological Tissue
Germ Cells
lcsh:Q
Transcriptome
Developmental Biology
Transcription Factors
Zdroj: PLoS ONE, Vol 13, Iss 1, p e0190800 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: The transcription factor MAFB is an important regulator of the development and differentiation of various organs and tissues. Previous studies have shown that MAFB is expressed in embryonic and adult mouse testes and is expected to act as the downstream target of retinoic acid (RA) to initiate spermatogenesis. However, its exact localization and function remain unclear. Here, we localized MAFB expression in embryonic and adult testes and analyzed its gene function using Mafb-deficient mice. We found that MAFB and c-MAF are the only large MAF transcription factors expressed in testes, while MAFA and NRL are not. MAFB was localized in Leydig and Sertoli cells at embryonic day (E) 18.5 but in Leydig cells, Sertoli cells, and pachytene spermatocytes in adults. Mafb-deficient testes at E18.5 showed fully formed seminiferous tubules with no abnormal structure or differences in testicular somatic cell numbers compared with those of control wild-type mice. Additionally, the expression levels of genes related to development and function of testicular cells were unchanged between genotypes. In adults, the expression of MAFB in Sertoli cells was shown to be stage specific and induced by RA. By generating Mafbfl/fl CAG-CreER™ (Mafb-cKO) mice, in which Cre recombinase was activated upon tamoxifen treatment, we found that the neonatal cKO mice died shortly upon Mafb deletion, but adult cKO mice were alive upon deletion. Adult cKO mice were fertile, and spermatogenesis maintenance was normal, as indicated by histological analysis, hormone levels, and germ cell stage-specific markers. Moreover, there were no differences in the proportion of seminiferous stages between cKO mice and controls. However, RNA-Seq analysis of cKO Sertoli cells revealed that the down-regulated genes were related to immune function and phagocytosis activity but not spermatogenesis. In conclusion, we found that MAFB is dispensable for fetal testis morphogenesis and spermatogenesis maintenance in adult mice, despite the significant gene expression in different cell types, but MAFB might be critical for phagocytosis activity of Sertoli cells.
Databáze: OpenAIRE
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