Down-Regulation of the Proteoglycan Decorin Fills in the Tumor-Promoting Phenotype of Ionizing Radiation-Induced Senescent Human Breast Stromal Fibroblasts
| Autor: | Petros N. Panagiotou, Heba Bassiony, Andreas M Yiacoumettis, Asimina Fotopoulou, Stathis Tsimelis, Harris Pratsinis, Adamantia Papadopoulou, Dimitris Kletsas, Eleni Mavrogonatou |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research autophagy senescence Decorin Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Breast cancer Stroma Downregulation and upregulation human breast stromal fibroblasts growth factors medicine Autocrine signalling RC254-282 decorin biology Chemistry Autophagy Neoplasms. Tumors. Oncology. Including cancer and carcinogens γ-irradiation medicine.disease VEGF carbohydrates (lipids) 030104 developmental biology Oncology Proteoglycan bFGF 030220 oncology & carcinogenesis biology.protein Cancer research breast cancer cells |
| Zdroj: | Cancers Cancers, Vol 13, Iss 1987, p 1987 (2021) Volume 13 Issue 8 |
| ISSN: | 2072-6694 |
| Popis: | Simple Summary Ionizing radiation (a typical remedy for breast cancer) results in the premature senescence of the adjacent to the neoplastic cells stromal fibroblasts. Here, we showed that these senescent fibroblasts are characterized by the down-regulation of the small leucine-rich proteoglycan decorin, a poor prognostic factor for the progression of the disease. Decorin down-regulation is mediated by secreted growth factors in an autocrine and paracrine (due to the interaction with breast cancer cells) manner, with bFGF and VEGF being the key players of this regulation in young and senescent breast stromal fibroblasts. Autophagy activation increases decorin mRNA levels, indicating that impaired autophagy is implicated in the reduction in decorin in this cell model. Decorin down-regulation acts additively to the already tumor-promoting phenotype of ionizing radiation-induced prematurely senescent human stromal fibroblasts, confirming that stromal senescence is a side-effect of radiotherapy that should be taken into account in the design of anticancer treatments. Abstract Down-regulation of the small leucine-rich proteoglycan decorin in the stroma is considered a poor prognostic factor for breast cancer progression. Ionizing radiation, an established treatment for breast cancer, provokes the premature senescence of the adjacent to the tumor stromal fibroblasts. Here, we showed that senescent human breast stromal fibroblasts are characterized by the down-regulation of decorin at the mRNA and protein level, as well as by its decreased deposition in the pericellular extracellular matrix in vitro. Senescence-associated decorin down-regulation is a long-lasting process rather than an immediate response to γ-irradiation. Growth factors were demonstrated to participate in an autocrine manner in decorin down-regulation, with bFGF and VEGF being the critical mediators of the phenomenon. Autophagy inhibition by chloroquine reduced decorin mRNA levels, while autophagy activation using the mTOR inhibitor rapamycin enhanced decorin transcription. Interestingly, the secretome from a series of both untreated and irradiated human breast cancer cell lines with different molecular profiles inhibited decorin expression in young and senescent stromal fibroblasts, which was annulled by SU5402, a bFGF and VEGF inhibitor. The novel phenotypic trait of senescent human breast stromal fibroblasts revealed here is added to their already described cancer-promoting role via the formation of a tumor-permissive environment. |
| Databáze: | OpenAIRE |
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