Structural perturbation by arsenic triggers the aggregation of hen egg white lysozyme by promoting oligomers formation
| Autor: | Vijay Kumar Ravi, Inderbhan Singh, M. S. Dahiya, Neelakant Varma, Satish Kumar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Beta sheet chemistry.chemical_element Protein aggregation Microscopy Atomic Force Models Biological Biochemistry Arsenic Protein Aggregates 03 medical and health sciences chemistry.chemical_compound Structural Biology Animals Arsenic trioxide Molecular Biology Incubation 030102 biochemistry & molecular biology Circular Dichroism General Medicine Dynamic Light Scattering In vitro 030104 developmental biology chemistry Hydrodynamics Muramidase Protein folding Protein Multimerization Lysozyme Protein Binding |
| Zdroj: | International Journal of Biological Macromolecules. 109:1108-1114 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.11.096 |
| Popis: | Arsenic trioxide is one of the most common metallic pollutants entering the food chain both by human activities and nature. Its entry inside the living organism through food, air and water results into the accumulation of heavy metal in several tissues which manifest several metabolic or hormonal disorders. Till now the effect of arsenic trioxide on protein misfolding and aggregation culminating into several neurodegenerative disorders is poorly understood. In the present study, we reveal the aggregation process of Hen Egg White Lysozyme (HEWL) in presence of arsenic trioxide (As2O3) at physiological conditions. We show that As2O3 promote the in vitro aggregation of HEWL in concentration dependent manner. Early phase of aggregation is observed to be induced by exposure of hydrophobic surfaces which later reorganized to promote further self-association leading to β sheet structure. Presence of lower ordered oligomers after two days and higher ordered oligomers along with amorphous aggregates after week long incubation indicate that As2O3 drives the self-assembly of lysozyme towards oligomeric form. |
| Databáze: | OpenAIRE |
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