Evolution and spread of Venezuelan equine encephalitis complex alphavirus in the Americas
| Autor: | Jyotsna Pandya, Timothy B. Stockwell, Rodion Gorchakov, Scott C. Weaver, Rebecca A. Halpin, Naomi L. Forrester, Grace Leal, Ravi Jain, Kumar Hari, David E. Wentworth, David Yin-wei Lin, Rubing Chen, Joel O. Wertheim, M. D. Smith, Albert J. Auguste, Jose G. Estrada-Franco, Vivian G. Dugan, A. Paige Adams, Sergei L. Kosakovsky Pond, Suman R. Das |
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| Přispěvatelé: | Caccone, Adalgisa |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
viruses Disease Vectors Q1 Mosquitoes Medical and Health Sciences Animal Diseases Encephalitis Virus Venezuelan Equine Database and Informatics Methods 0302 clinical medicine Medicine and Health Sciences 2.2 Factors relating to the physical environment Aetiology Clade Molecular clock Encephalomyelitis Phylogeny Data Management Genetics Mammalian Genomics Phylogenetic tree lcsh:Public aspects of medicine virus diseases R735 Encephalomyelitis Venezuelan Equine Phylogenetic Analysis Genomics Biological Sciences Genomic Databases 3. Good health Insects Phylogenetics Culex Infectious Diseases Viral evolution Enzootic Infection Venezuelan Equine Encephalitis Sequence Analysis Research Article Biotechnology Computer and Information Sciences lcsh:Arctic medicine. Tropical medicine Arthropoda lcsh:RC955-962 Bioinformatics Evolution 030231 tropical medicine Venezuelan Equine Alphavirus Biology Research and Analysis Methods Microbiology Viral Evolution Evolution Molecular Vaccine Related 03 medical and health sciences Virology Biodefense Tropical Medicine Animals Humans Evolutionary Systematics Horses Amino Acid Sequence Taxonomy Evolutionary Biology Sequence Assembly Tools Prevention Public Health Environmental and Occupational Health Organisms Biology and Life Sciences Computational Biology Molecular lcsh:RA1-1270 Encephalitis Virus biology.organism_classification Genome Analysis Invertebrates R1 Organismal Evolution Insect Vectors Vector-Borne Diseases Species Interactions 030104 developmental biology Biological Databases Emerging Infectious Diseases Animal Genomics Microbial Evolution Horse Diseases Americas Zoology Sequence Alignment |
| Zdroj: | PLoS neglected tropical diseases, vol 11, iss 8 PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Vol 11, Iss 8, p e0005693 (2017) |
| ISSN: | 1935-2735 |
| Popis: | Venezuelan equine encephalitis (VEE) complex alphaviruses are important re-emerging arboviruses that cause life-threatening disease in equids during epizootics as well as spillover human infections. We conducted a comprehensive analysis of VEE complex alphaviruses by sequencing the genomes of 94 strains and performing phylogenetic analyses of 130 isolates using complete open reading frames for the nonstructural and structural polyproteins. Our analyses confirmed purifying selection as a major mechanism influencing the evolution of these viruses as well as a confounding factor in molecular clock dating of ancestors. Times to most recent common ancestors (tMRCAs) could be robustly estimated only for the more recently diverged subtypes; the tMRCA of the ID/IAB/IC/II and IE clades of VEE virus (VEEV) were estimated at ca. 149–973 years ago. Evolution of the IE subtype has been characterized by a significant evolutionary shift from the rest of the VEEV complex, with an increase in structural protein substitutions that are unique to this group, possibly reflecting adaptation to its unique enzootic mosquito vector Culex (Melanoconion) taeniopus. Our inferred tree topologies suggest that VEEV is maintained primarily in situ, with only occasional spread to neighboring countries, probably reflecting the limited mobility of rodent hosts and mosquito vectors. Author summary The Venezuelan equine encephalitis (VEE) complex comprises a broadly distributed group of alphaviruses in the Americas that have the potential to emerge and cause severe disease. Historically, VEE complex viruses have caused recurring outbreaks of human and equine encephalitis in Central and South America as well as Mexico, with at least one outbreak resulting in movement of the virus to the southern United States. We present the most comprehensive phylogenetic analysis of complete genomic sequences of the most prominent member of the VEE complex, VEE virus (VEEV). We were able to identify the major forces influencing VEEV evolution, and using the inferred phylogenies we determined that VEEV evolves in geographically segregated lineages with enzootic transmission between rodents and mosquitoes apparently limiting its spread. |
| Databáze: | OpenAIRE |
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