Methyl jasmonate treatment increases podophyllotoxin production in Podophyllum hexandrum roots under glasshouse conditions
| Autor: | Rita Setroikromo, Peter Horvatovich, Ron Peters, Christel L. C. Seegers, Pieter G. Tepper, Wim J. Quax |
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| Přispěvatelé: | Chemical and Pharmaceutical Biology, Analytical Biochemistry, Medicinal Chemistry and Bioanalysis (MCB), Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Nanotechnology and Biophysics in Medicine (NANOBIOMED) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine ALKALOID BIOSYNTHESIS Soil Science Greenhouse Plant Science PROPAGATION Podophyllum hexandrum Methyl jasmonate 01 natural sciences SECONDARY METABOLISM 03 medical and health sciences chemistry.chemical_compound Soil DIFFERENT POPULATIONS Botany medicine ARYLTETRALIN LIGNANS CELL-CULTURES PELTATUM PLANT Secondary metabolism Etoposide Podophyllotoxin biology Podophyllum Temperature Plant physiology biology.organism_classification 030104 developmental biology YIELD chemistry HIMALAYAN REGION 010606 plant biology & botany Teniposide medicine.drug |
| Zdroj: | Plant and Soil, 417(1-2), 117-126. SPRINGER |
| ISSN: | 1573-5036 |
| Popis: | The endangered Podophyllum hexandrum is an important industrial source of podophyllotoxin, which is a precursor for the anticancer drugs etoposide and teniposide. Attempts to obtain podophyllotoxin through cell cultures or chemical synthesis have still a long way to go before being economical feasible. The objective of this study was to increase the root formation and podophyllotoxin production of P. hexandrum cultivated in a glasshouse.Root formation and podophyllotoxin production of P. hexandrum in sand or peat-perlite soil at 15 A degrees C or 25 A degrees C was determined. Furthermore, the influence of methyl jasmonate on the podophyllotoxin production was determined.More root formation was observed in peat-perlite soil than in sand soil. Furthermore, root formation was higher at 15 A degrees C than at 25 A degrees C. This resulted in the highest podophyllotoxin production per plant in peat-perlite at 15 A degrees C (160 +/- 22 mg/plant d.w.). Furthermore, methyl jasmonate treatment of the leaves increased the podophyllotoxin production in the roots by 21%.We were able to cultivate P. hexandrum in a glasshouse in the Netherlands and improve the root formation and podophyllotoxin production. This paves the way for large-scale cultivation of P. hexandrum in the temperate latitudes for the production of the pharmaceutical interesting podophyllotoxin. |
| Databáze: | OpenAIRE |
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