Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode
| Autor: | Miguel D. Toscano, Antonio L. De Lacey, Marcos Pita, Cristina Gutierrez-Sanchez, Sergey Shleev |
|---|---|
| Přispěvatelé: | European Commission, Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2013 |
| Předmět: |
Oxidoreductases Acting on CH-CH Group Donors
Inorganic chemistry Biophysics chemistry.chemical_element Biosensing Techniques macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Oxygen Bilirubin oxidase Electrochemistry Gold nanoparticles Humans Organic chemistry Physical and Theoretical Chemistry Electrodes Detection limit Chemistry technology industry and agriculture General Medicine Chronoamperometry 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences Biosensors Immobilized Proteins Linear range Direct electron transfer Covalent bond Colloidal gold Biocatalysis Gold 0210 nano-technology Biosensor |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2013.07.001 |
| Popis: | Gold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-less biosensors for oxygen in a buffer that mimics the content and the composition of human physiological fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6 ± 1 μM with a linear range of 6-300 μM, i.e. exceeding usual physiological ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase. This work was funded by the FP7 project “3D-Nanobiodevice” (NMP4-SL-2009-229255). We thank Novozymes A/S for the preparation of Myrothecium verrucaria BOx. M.P. acknowledges the 2009 Ramon y Cajal program from the Spanish MINECO. |
| Databáze: | OpenAIRE |
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