The endoplasmic reticulum is the site of cholesterol-induced cytotoxicity in macrophages
| Autor: | David Ron, Cecilia Devlin, Bo Feng, Dajun Zhang, Michele Sweeney, Ira Tabas, Yankun Li, Andrew R. Marks, Heather P. Harding, Edward A. Fisher, George Kuriakose, Pin Mei Yao, James X. Rong |
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| Rok vydání: | 2003 |
| Předmět: |
Programmed cell death
Protein Folding Biological Transport Active Apoptosis Coronary Artery Disease Biology CHOP Endoplasmic Reticulum Mice Animals Calcium Signaling Cells Cultured Calcium signaling Transcription Factor CHOP Mice Knockout Endoplasmic reticulum Macrophages STIM1 Cell Biology Intracellular Membranes Cell biology Cholesterol Unfolded protein response CCAAT-Enhancer-Binding Proteins Female biological phenomena cell phenomena and immunity Signal Transduction Transcription Factors |
| Zdroj: | Nature cell biology. 5(9) |
| ISSN: | 1465-7392 |
| Popis: | Excess cellular cholesterol induces apoptosis in macrophages, an event likely to promote progression of atherosclerosis. The cellular mechanism of cholesterol-induced apoptosis is unknown but had previously been thought to involve the plasma membrane. Here we report that the unfolded protein response (UPR) in the endoplasmic reticulum is activated in cholesterol-loaded macrophages, resulting in expression of the cell death effector CHOP. Cholesterol loading depletes endoplasmic reticulum calcium stores, an event known to induce the UPR. Furthermore, endoplasmic reticulum calcium depletion, the UPR, caspase-3 activation and apoptosis are markedly inhibited by selective inhibition of cholesterol trafficking to the endoplasmic reticulum, and Chop-/- macrophages are protected from cholesterol-induced apoptosis. We propose that cholesterol trafficking to endoplasmic reticulum membranes, resulting in activation of the CHOP arm of the UPR, is the key signalling step in cholesterol-induced apoptosis in macrophages. |
| Databáze: | OpenAIRE |
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