Evidence That p-Cresol and IL-6 Are Adsorbed by the HFR Cartridge: Towards a New Strategy to Decrease Systemic Inflammation in Dialyzed Patients?
| Autor: | Bruno Memoli, Eleonora Riccio, Gennaro Argentino, Roberta Russo, Mauro Cataldi, Maristella Minco, Lucia Grumetto, Bruna Guida, Stefania Brancaccio, Loredana Postiglione, Andrea Memoli |
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| Přispěvatelé: | Riccio, Eleonora, Cataldi, Mauro, Minco, Maristella, Argentino, Gennaro, Russo, Roberta, Brancaccio, Stefania, Memoli, Andrea, Grumetto, Lucia, Postiglione, Loredana, Guida, Bruna, Memoli, Bruno |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_treatment lcsh:Medicine Systemic inflammation Toxicology Vascular Medicine Cresols Blood plasma Chronic Kidney Disease Toxin Binding Medicine and Health Sciences p-Cresol lcsh:Science Multidisciplinary biology Dialysi Middle Aged Nephrology Female Hemodialysis medicine.symptom Research Article Biotechnology medicine.medical_specialty Serum albumin Cardiology Inflammation Hemodiafiltration Peripheral blood mononuclear cell End stage renal disease Hfr cell Internal medicine parasitic diseases Medical Dialysis medicine Humans Serum Albumin Aged IL-6 business.industry Interleukin-6 lcsh:R Biology and Life Sciences Atherosclerosis Hemodialysis Solutions Surgery Endocrinology biology.protein Kidney Failure Chronic lcsh:Q Medical Devices and Equipment Adsorption Clinical Medicine business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 4, p e95811 (2014) |
| ISSN: | 1932-6203 0186-5773 |
| Popis: | Introduction Hemodialysis (HD) and hemodiafiltration clear only with a low efficiency the plasma from interleukin-6 and p-cresol, two protein-bound uremic toxins associated with high cardiovascular risk in end stage renal disease. HFR Supra is a double-chamber hemodiafiltration system in which the ultrafiltrate returns to the patient after its regeneration through a resin cartridge that binds hydrophobic and protein-bound solutes. In the present study, we evaluated whether the HFR cartridge can also bind total p-cresol and IL-6 and remove them from the ultrafiltrate. Methods We compared the levels of IL-6 and p-cresol in ultrafiltrate samples collected at the inlet (UFin) and at the outlet (UFout) of the cartridge at the start or at the end of a 240 min HFR session in 12 inflamed chronic HD patients. The pro-inflammatory activity of the ultrafiltrate samples was also determined by evaluating the changes that they induced in IL-6 mRNA expression and protein release in peripheral blood mononuclear cells from 12 healthy volunteers. IL-6 and p-cresol circulating levels were also assessed in peripheral plasma blood samples collected before and after HFR and, for comparison, a control HD. Results p-Cresol and IL-6 were lower in UFout than in UFin both at the start and at the end of the HFR session, suggesting that they were retained by the cartridge. IL-6 mRNA expression and release were lower in PBMC incubated with UFout collected at the end than with UFin collected at the start of HFR, suggesting that passage through the cartridge reduced UF pro-inflammatory activity. Plasma total p-cresol decreased by about 53% after HFR, and 37% after HD. IL-6 circulating values were unmodified by either these dialysis procedures. Conclusions This study shows that the HFR-Supra cartridge retains total p-cresol and IL-6 in the ultrafiltrate and lowers plasma total p cresol but not IL-6 levels. Trial Registration ClinicalTrials.gov NCT01865773 |
| Databáze: | OpenAIRE |
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