Robust and simple interface for microchip electrophoresis–mass spectrometry
| Autor: | Akihiro Arai, Shin Nakamura, Koji Otsuka, Yuji Tachibana, Shigeru Terabe, Koichi Suzuki |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
Electrospray Chromatography Rhodamines Chemistry Organic Chemistry Microfluidics Analytical chemistry General Medicine Mass spectrometry Biochemistry Mass Spectrometry Buffer (optical fiber) Analytical Chemistry Spray nozzle Polyether ether ketone chemistry.chemical_compound Electrophoresis Capillary electrophoresis Pharmaceutical Preparations Semiconductors |
| Zdroj: | Journal of Chromatography A. 1011:181-192 |
| ISSN: | 0021-9673 |
| Popis: | A robust and simple interface for microchip electrophoresis-mass spectrometry (MCE-MS) was developed using a spray nozzle connected to the exit of the separation channel of the microchip. The spray nozzle was attached to the microchip using a polyether ether ketone screw without adhesive, thus allowing easy replaced. Sample injection and electrophoretic separation was performed by control of the voltage only. The analysis of a few basic drugs was performed using the optimized MCE-MS system. The separation was improved by using a high-viscosity separation buffer and a spray nozzle with a small bore size. This system was also applied to the separation of peptides and protein-trypsin digests. Sample adsorption was minimized by adding acetonitrile to the separation buffer when using a quartz microchip. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect