Ribosome nascent chain complexes of the chloroplast-encoded cytochrome b6 thylakoid membrane protein interact with cpSRP54 but not with cpSecY
| Autor: | Bożena Króliczewska, Małgorzata Piskozub, Jarosław Króliczewski |
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| Rok vydání: | 2014 |
| Předmět: |
Protein Folding
Chloroplasts Physiology Blotting Western Biology cpSRP54 Thylakoids Mass Spectrometry Article Thylakoid membrane Chloroplast ribosome Chloroplast Proteins Cytochrome C1 Immunoprecipitation Signal recognition particle Cytochrome b Arabidopsis Proteins Cytochrome c cpSecY Peas Membrane Proteins Translation (biology) Cytochrome b6 Cell Biology Cross-Linking Reagents Biochemistry Cytochromes b6 Thylakoid Protein Biosynthesis Membrane protein Biophysics biology.protein Quinolines Protein folding Electrophoresis Polyacrylamide Gel RNC Ribosomes Signal Recognition Particle SEC Translocation Channels |
| Zdroj: | Journal of Bioenergetics and Biomembranes |
| ISSN: | 1573-6881 |
| Popis: | We analysed the interplay between the cpSecY, cpSRP54 and the chloroplast-encoded cytochrome b6 via isolation of chloroplast ribosome nascent chain complexes and the use of cross-linking factors, antibodies and mass spectroscopy analyses. We showed that the cytochrome b6 nascent polypeptide complex is tightly associated with ribosomes and that the translation of cytochrome b6 was discontinuous. The causes of ribosome pausing and the functional significance of this phenomenon may be related to proper protein folding, insertion into thylakoid membranes and the association of cofactors during this process. It was also found that cpSecY was not in the vicinity of cytochrome b6 intermediates during the elongation process and does not act with mature cytochrome b6 after translation. Using the approach of cross-linking during elongation of the cytochrome b6 protein, we showed that cpSRP54 interacts strongly with the elongating nascent chain. Electronic supplementary material The online version of this article (doi:10.1007/s10863-014-9598-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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