Fabrication, Characterization and Application of Biomolecule Micropatterns on Cyclic Olefin Polymer (COP) Surfaces with Adjustable Contrast

Autor: Anna-Maria Lipp, Roland Hager, Sandra Haas, Julian Weghuber, Thomas Haselgrübler
Rok vydání: 2019
Předmět:
Streptavidin
micropatterned polymers
Fabrication
Materials science
Polymers
Surface Properties
lcsh:Biotechnology
micropatterns
Clinical Biochemistry
Nanotechnology
Biosensing Techniques
02 engineering and technology
010402 general chemistry
01 natural sciences
Article
law.invention
Jurkat Cells
chemistry.chemical_compound
law
lcsh:TP248.13-248.65
Microscopy
Humans
photolithography
Cells
Cultured
total internal reflection fluorescence (TIRF)
chemistry.chemical_classification
Total internal reflection fluorescence microscope
Biomolecule
Plasma activation
Optical Imaging
Cycloparaffins
General Medicine
021001 nanoscience & nanotechnology
0104 chemical sciences
cyclic olefin polymer
protein–protein interaction
Microscopy
Fluorescence
chemistry
Microcontact printing
Photolithography
0210 nano-technology
Zdroj: Biosensors
Volume 10
Issue 1
Biosensors, Vol 10, Iss 1, p 3 (2019)
ISSN: 2079-6374
DOI: 10.3390/bios10010003
Popis: Peptide and protein micropatterns are powerful tools for the investigation of various cellular processes, including protein&ndash
protein interactions (PPIs). Within recent years, various approaches for the production of functional surfaces have been developed. Most of these systems use glass as a substrate, which has several drawbacks, including high fragility and costs, especially if implemented for fluorescence microscopy. In addition, conventional fabrication technologies such as microcontact printing (µ
CP) are frequently used for the transfer of biomolecules to the glass surface. In this case, it is challenging to adjust the biomolecule density. Here, we show that cyclic olefin polymer (COP) foils, with their encouraging properties, including the ease of manufacturing, chemical resistance, biocompatibility, low water absorption, and optical clarity, are a promising alternative to glass substrates for the fabrication of micropatterns. Using a photolithography-based approach, we generated streptavidin/biotinylated antibody patterns on COPs with the possibility of adjusting the pattern contrast by varying plasma activation parameters. Our experimental setup was finally successfully implemented for the analysis of PPIs in the membranes of live cells via total internal reflection fluorescence (TIRF) microscopy.
Databáze: OpenAIRE
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