Microstructured Reactors Designed by Stereolithography and Characterized by Fluorescent Probes
| Autor: | F. Evenou, Nadine Martinet, M. C. Carré, F. Baros, Serge Corbel, M. Donner |
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| Přispěvatelé: | Département de Chimie Physique des Réactions (DCPR), Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS), Biologie Cellulaire et Moleculaire du Transport des Nutriments, Université Henri Poincaré - Nancy 1 (UHP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Materials science
Article Subject lcsh:TJ807-830 lcsh:Renewable energy sources Nanotechnology 02 engineering and technology Photoresist 010402 general chemistry fluorescence microscopy 01 natural sciences Fluorescence spectroscopy law.invention fluorescent probes law Microscopy Résine SU-8 5 General Materials Science Stereolithography covalent grafting Renewable Energy Sustainability and the Environment General Chemistry 021001 nanoscience & nanotechnology Grafting stereolithography Fluorescence Atomic and Molecular Physics and Optics laser 0104 chemical sciences Polymerization Covalent bond photopolymerization microwells [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] 0210 nano-technology |
| Zdroj: | International Journal of Photoenergy, Vol 2008 (2008) International Journal of Photoenergy International Journal of Photoenergy, Hindawi Publishing Corporation, 2008, Article ID 757510, 7 p. ⟨10.1155/2008/757510⟩ |
| ISSN: | 1110-662X |
| DOI: | 10.1155/2008/757510 |
| Popis: | International audience; The main objective of this research was to define a structured and functionalized support for future biomedical applications (model of “low-density bioarray”). The experiments were carried out by using stereolithography process with a special SU-8 photoresist and the reproducibility of the method was studied by analyzing the surface profile of the support. Finally, a matrix of regular controlled sized wells was fabricated. Chemical reactions leading to covalent grafting were run to demonstrate that the inner surface of the wells remains still reactive after polymerization. The grafting of fluorophores with carboxylic functions activated by N-hydroxysuccinimide was studied as function of time, in order to determine the best reactions, conditions. Then, the grafting of two distinct fluorescent probes was led simultaneously inside the wells, showing the possibility of spatial localization of diverse reactions on the same support. The covalent and localized bindings were confirmed by fluorescence spectroscopy and microscopy analyses. |
| Databáze: | OpenAIRE |
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