| Autor: |
Kaj Henriksen, Peter Roslev, Torben Olesen, Dennis E. Rolston, Martin Hesselsøe, Jesper Gamst, Per Moldrup |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Gamst, J, Hesselsøe, M, Roslev, P, Roslev, P, Møldrup, P, Henriksen, K & Henriksen, K 2002, ' Quantification of 14C-labeled hydrophobic organic compounds in soil samples by a scintillation fluid extraction method ', Soil Science, vol. 167, no. 1, pp. 25-34 . |
| ISSN: |
0038-075X |
| DOI: |
10.1097/00010694-200201000-00003 |
| Popis: |
Hydrophobic organic chemicals labeled with 14 C are often used as tracers to study chemical fate and transport in soil. Wet oxidation is the recognized but labor-demanding method used most often to measure the concentration of radioactive organic tracers in the soil. In this study, we test an alternative, simpler method for quantification of 14 C-labeled hydrophobic organic chemicals in soil samples. The soil samples were extracted directly with scintillation fluid in glass scintillation vials (Scintillation Fluid Extraction, SFE method) and were subsequently analyzed by liquid scintillation counting. Application of internal standards showed no significant quenching or reduced counts of the 14 C-labeled chemical caused by the presence of settled soil particles in the scintillation vials. Hence, the scintillation fluid could be used simultaneously as both scintillation and extraction media. Decreasing the amount of soil containing 14 C-labeled chemicals added to the scintillation vial was shown to increase the extraction efficiency of the SFE method. Aging (contact time) and concentration were shown to affect the results of the SFE method. However, in a naphthalene diffusion experiment, where aging varied between almost no contact time and 20 days and concentration of naphthalene varied between ∼0 to 40 μg g -1 , the estimated naphthalene diffusion coefficient was shown to be influenced only minimally when using the SFE method compared with the wet oxidation method. We emphasize that the SFE method is applicable only to sterile systems with no degradation or assimilation of 14 C-labeled compounds. If this condition is met and there is appropriate consideration of the effects of chemical aging and concentration, the SFE method seems to be a useful and labor-saving alternative to the traditional wet oxidation method for determination of the concentration of 14 C-labeled organic chemicals in soil samples. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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