Simultaneous determination of ten nonsteroidal anti-inflammatory drugs from drinking water, surface water and wastewater using micro UHPLC-MS/MS with on-line SPE system
| Autor: | Balázs Magda, Zoltán Márta, Jenő Fekete, Tímea Imre, Balázs Bobály, Pál Szabó |
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| Rok vydání: | 2018 |
| Předmět: |
Formic acid
Clinical Biochemistry Pharmaceutical Science 010501 environmental sciences Wastewater Mass spectrometry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Drug Discovery medicine Sample preparation Solid phase extraction Spectroscopy Chromatography High Pressure Liquid 0105 earth and related environmental sciences Detection limit Fenoprofen Chromatography Drinking Water 010401 analytical chemistry Anti-Inflammatory Agents Non-Steroidal Solid Phase Extraction Purified water 0104 chemical sciences Dilution chemistry Water Pollutants Chemical medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 160 |
| ISSN: | 1873-264X |
| Popis: | A simple, accurate and sensitive micro UHPLC-MS/MS method was developed and validated for the simultaneous determination of 10 nonsteroidal anti-inflammatory drugs (NSAIDs) from different environmental matrices. The micro LC ‒ on-line SPE method described in this study allowed to determine the selected drugs at ultra-trace levels without the most commonly used complex off-line SPE sample preparation procedures. The presented method is capable of reaching satisfactory low LOQ values with analysing the sample directly after being diluted with water. In order to attain high sensitivity, mass spectrometry was carefully optimized for the analysis of the drugs. Fenoprofen, flurbiprofen and naproxen were found to produce CO2 loss during ionization, forming intense [M-H-CO2]- ions instead of [M-H]-. All the other compounds were analyzed through their [M+H]+ and [M-H]- ions. Effect of mobile phase pH on ionization was also studied. Lower pH resulted in higher ion intensities. For this reason, a reversed phase chromatographic separation was applied at pH 3.1 with formic acid at concentration of 0.01%. Matrix effects have been evaluated during validation and sample dilution was optimized focusing on the lowest achievable LOQ values. Analytes were determined from drinking water directly, from surface water and wastewater following dilution with purified water by 2 : 8 (v/v) and 1 : 9 (v/v), respectively. Finally, the method was applied to real sample analysis. |
| Databáze: | OpenAIRE |
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