Preparation of a monoclonal antibody and development of an indirect competitive ELISA for the detection of chlorpromazine residue in chicken and swine liver
| Autor: | Shanliang Wang, Yuping Wan, Caiwei Feng, Weiwei Yin, Rimo Xi, Weihua Li, Wei Liu, Meng Meng |
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| Rok vydání: | 2010 |
| Předmět: |
Drug
Meat Immunogen Chlorpromazine Swine medicine.drug_class media_common.quotation_subject Enzyme-Linked Immunosorbent Assay Food Contamination Monoclonal antibody Inhibitory Concentration 50 medicine Animals Bovine serum albumin IC50 media_common Nutrition and Dietetics Chromatography medicine.diagnostic_test biology Antibodies Monoclonal Reproducibility of Results Serum Albumin Bovine Liver Immunoassay biology.protein Cattle Antibody Chickens Agronomy and Crop Science Food Science Biotechnology medicine.drug |
| Zdroj: | Journal of the Science of Food and Agriculture. |
| ISSN: | 1097-0010 0022-5142 |
| DOI: | 10.1002/jsfa.4014 |
| Popis: | BACKGROUND: Chlorpromazine is a typical antipsychotic drug used to make food-producing animals calm and promote growth as feed additives. Accumulation of chlorpromazine in animal bodies would cause side effects in the circulatory and nervous systems, and have adverse effects on blood cells, the skin and the eye. To detect the chlorpromazine residue in food producing animals, an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed based on preparation of an anti-chlorpromazine monoclonal antibody. RESULTS: The antibody generated from immunogen of cationic bovine serum albumin (cBSA) coupled with chlorpromazine showed high sensitivity toward chlorpromazine with an IC50 value of 0.73 ppb. The ELISA method was applied to detect swine liver and chicken samples spiked by chlorpromazine and satisfactory results were obtained. The recovery rates in chicken and swine liver were in the range of 88–95% and 86–95%, respectively; the intra-assay coefficients of variation were both < 15.3% and < 13.5%, respectively. CONCLUSION: An indirect competitive ELISA method based on a monoclonal antibody towards chlorpromazine with excellent sensitivity and specificity has been successfully developed. The immunoassay provided in this study was a hopeful alternative to chromatography spectrometry for regulatory analysis of chlorpromazine residue in food-producing animals. Copyright © 2010 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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