EVALUATION AND SIGNIFICANCE OF TETRACYCLINE STABILITY IN RABIES VACCINE BAITS
| Autor: | T. Buettgenbach, John J. Johnston, Carol A. Furcolow, Dennis Slate, Richard B. Chipman, Thomas J. DeLiberto, Margaret J. Goodall, Thomas M. Primus, J. L. Snow |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Veterinary medicine
Ecology Rabies Tetracycline Administration Oral food and beverages Animals Wild macromolecular substances Biology medicine.disease Intestinal absorption Distribution chain Rabies vaccine Intestinal Absorption Microscopy Fluorescence Rabies Vaccines parasitic diseases medicine Animals Detection rate Biomarkers Ecology Evolution Behavior and Systematics medicine.drug |
| Zdroj: | Journal of Wildlife Diseases. 41:549-558 |
| ISSN: | 0090-3558 |
| DOI: | 10.7589/0090-3558-41.3.549 |
| Popis: | Tetracycline is widely used as a biomarker for bait consumption by wildlife; tetra- cycline is incorporated into bones and teeth and can be detected by fluorescence microscopy several weeks postconsumption. During 2003, the United States Department of Agriculture dis- tributed more than 10 million tetracycline-containing rabies-vaccine baits to control the spread of wildlife vectored rabies to humans, pets, and livestock. To estimate the percentage of target species consuming the baits, raccoons and skunks were collected in baited areas and teeth were analyzed for the presence of the biomarker. Several incidents of low biomarker detection rates prompted an investigation of the stability of the biomarker in the baits. Baits were collected at several points along the manufacturing and distribution chain. Baits were analyzed for free and polymer-bound tetracycline and the less active isomer epitetracycline. Results indicated that a portion of the tetracycline was converted to epitetracycline. Additionally, significant quantities of both compounds were trapped in the polymer, which is homogeneously distributed throughout the bait. The results of this study suggest that approximately 40% of the target quantity of tet- racycline was unavailable for absorption. This situation could contribute to low biomarker detec- tion rates and suggests that formulation modification should be considered. |
| Databáze: | OpenAIRE |
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