Coupling astogenic aging in the colonial tunicate Botryllus schlosseri with the stress protein mortalin
| Autor: | Baruch Rinkevich, Claudette Rabinowitz, Oshrat Ben-Hamo, Amalia Rosner, Matan Oren |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Senescence Aging Zooid Somatic cell Pyridines Apoptosis Botryllus schlosseri 03 medical and health sciences Reproduction Asexual Animals HSP70 Heat-Shock Proteins Urochordata Molecular Biology Heat-Shock Proteins HSPA9 biology Botryllus Age Factors Cell Biology biology.organism_classification Cell biology Tunicate Thiazoles 030104 developmental biology Immunology Developmental Biology Endostyle |
| Zdroj: | Developmental biology. 433(1) |
| ISSN: | 1095-564X |
| Popis: | Botryllus schlosseri, a colonial marine invertebrate, exhibits three generations of short-lived astogenic modules that continuously grow and die throughout the colony's entire lifespan, within week-long repeating budding cycles (blastogenesis), each consisting of four stages (A-D). At stage D, aging is followed by the complete absorption of adult modules (zooids) via a massive apoptotic process. Here we studied in Botryllus the protein mortalin (HSP70s member), a molecule largely known for its association with aging and proliferation. In-situ hybridization and qPCR assays reveal that mortalin follows the cyclic pattern of blastogenesis. Colonies at blastogenic stage D display the highest mortalin levels, and young modules exhibit elevated mortalin levels compared to old modules. Manipulations of mortalin with the specific allosteric inhibitor MKT-077 has led to a decrease in the modules' growth rate and the development of abnormal somatic/germinal morphologies (primarily in vasculature and in organs such as the endostyle, the stomach and gonads). We therefore propose that mortalin plays a significant role in the astogeny and aging of colonial modules in B. schlosseri, by direct involvement in the regulation of blastogenesis. |
| Databáze: | OpenAIRE |
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