Genetic characteristics and antimicrobial resistance of Staphylococcus epidermidis isolates from patients with catheter-related bloodstream infections and from colonized healthcare workers in a Belgian hospital

Autor: Soraya Cherifi, Carole Nagant, Baudouin Byl, Olivier Denis, Marie Hallin, Claire Nonhoff, Ariane Deplano
Rok vydání: 2014
Předmět:
health care facilities
manpower
and services
Bacteremia
Drug resistance
Catheter-related bloodstream infection
Medical microbiology
Belgium
Staphylococcus epidermidis
Bacteremia -- epidemiology -- microbiology
Pathologie maladies infectieuses
Cross Infection
Molecular Epidemiology
biology
Transmission (medicine)
virus diseases
food and beverages
PFGE
General Medicine
Staphylococcal Infections
Cross Infection -- epidemiology -- microbiology
Hospitals
Biofilms -- growth & development
Electrophoresis
Gel
Pulsed-Field
Microbiologie et protistologie [entomologie
phytoparasitolog.]
Catheter
Infectious Diseases
Carrier State
Virulence Factors -- genetics
MLST
Microbiologie et protistologie [parasitologie hum. et anim.]
Microbiology (medical)
medicine.medical_specialty
Genotype
Virulence Factors
Health Personnel
education
Staphylococcal infections
Microbiology
Antibiotic resistance
Drug Resistance
Bacterial
medicine
Belgium -- epidemiology
Humans
Staphylococcal Infections -- epidemiology -- microbiology
Research
fungi
Genetic Variation
biochemical phenomena
metabolism
and nutrition
Catheter-Related Infections -- epidemiology -- microbiology
medicine.disease
biology.organism_classification
Staphylococcus epidermidis -- classification -- drug effects -- genetics -- isolation & purification
Biofilms
Catheter-Related Infections
Carrier State -- epidemiology -- microbiology
Microbiologie et protistologie [bacteriol.virolog.mycolog.]
Multilocus Sequence Typing
Zdroj: Annals of Clinical Microbiology and Antimicrobials, 13 (1
Annals of Clinical Microbiology and Antimicrobials
ISSN: 1476-0711
DOI: 10.1186/1476-0711-13-20
Popis: Background: Staphylococcus epidermidis is a pathogen that is frequently encountered in the hospital environment. Healthcare workers (HCWs) can serve as a reservoir for the transmission of S. epidermidis to patients.Methods: The aim of this study was to compare and identify differences between S. epidermidis isolated from 20 patients with catheter-related bloodstream infections (CRBSIs) and from the hands of 42 HCWs in the same hospital in terms of antimicrobial resistance, biofilm production, presence of the intercellular adhesion (ica) operon and genetic diversity (pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCC) mec typing).Results: S. epidermidis isolates that caused CRBSI were resistant to significantly more non-betalactam drugs than were isolates collected from HCWs. Among the 43 mecA positive isolates (26 from HCWs), the most frequent SCCmec type was type IV (44%). The ica operon was significantly more prevalent in CRBSI isolates than in HCWs (P < 0.05). Weak in vitro biofilm production seemed to correlate with the absence of the ica operon regardless of the commensal or pathogenic origin of the isolate. The 62 isolates showed high diversity in their PFGE patterns divided into 37 different types: 19 harbored only by the CRBSI isolates and 6 shared by the clinical and HCW isolates. MLST revealed a total of ten different sequence types (ST). ST2 was limited to CRBSI-specific PFGE types while the " mixed" PFGE types were ST5, ST16, ST88 and ST153.Conclusion: One third of CRBSI episodes were due to isolates belonging to PFGE types that were also found on the hands of HCWs, suggesting that HCW serve as a reservoir for oxacillin resistance and transmission to patients. However, S. epidermidis ST2, mecA-positive and icaA-positive isolates, which caused the majority of clinically severe CRBSI, were not recovered from the HCW's hands. © 2014 Cherifi et al. licensee BioMed Central Ltd.
SCOPUS: ar.j
info:eu-repo/semantics/published
Databáze: OpenAIRE
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