Origanum vulgare essential oil affects pathogens causing vaginal infections
| Autor: | Jan Sudji, Mirjana Bogavac, Kristina Tešanović, Ljiljana Janjušević, Maja Karaman, B. Radovanović |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus 030106 microbiology Microbial Sensitivity Tests medicine.disease_cause Applied Microbiology and Biotechnology law.invention Microbiology 03 medical and health sciences Anti-Infective Agents law Origanum Candida albicans Escherichia coli Oils Volatile medicine Humans Plant Oils Bioassay Essential oil Candida biology Chemistry General Medicine Antimicrobial biology.organism_classification Corpus albicans 3. Good health Fungicide 030104 developmental biology Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 122:1177-1185 |
| ISSN: | 1364-5072 |
| Popis: | Aims This study aimed to investigate the application of Origanum vulgare L. essential oil (EO) as an alternative antimicrobial agent against vaginal infections. Methods and Results Microdilution methods were applied for the detection of the minimal inhibitory (MIC) and the minimal bactericidal/fungicidal (MBC/MFC) concentration of 15 clinical strains originating from women with symptoms of vaginal infection. Optical density determination (OD) was used for detection of the Candida growth rate. Staining with DAPI was used to detect the influence of EO on nuclear condensation and fragmentation, while the brine shrimp bioassay was used to determine the toxicity of EO. Chemical composition analysis was done using GS-MS. According to the MIC and MBC/MFC values, the most susceptible strains to EO were: Escherichia coli 1, E. coli 2, Staphylococcus aureus 3 and Candida albicans 1–3. Inhibition of C. albicans filamentation was detected at 0·45 μl ml−1. Conclusions The obtained inhibition (%) from Candida growth curves points to a shorter period of time (24 h) for determining IC50 as MIC and IC99 as MFC value. These values could be recommended as valid parameters for the faster detection of the effectiveness of EO on Candida isolates. Significance and Impact of the Study Examination of potential of the O. vulgare EO as a main antimicrobial constituent within vaginalettes in gynaecological practice. |
| Databáze: | OpenAIRE |
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