Induction of apoptosis by an inhibitor of EGFR in neuroblastoma cells
| Autor: | Shinichi Tamura, Yasumichi Kuwahara, Hajime Hosoi, Kunihiko Tsuchiya, Takahiro Gotoh, Moriatsu Izumi, Tohru Sugimoto, Ken Kikuchi, Osamu Otabe, Tomoko Iehara |
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| Rok vydání: | 2007 |
| Předmět: |
Cell
Biophysics Antineoplastic Agents Apoptosis Biochemistry Neuroblastoma Gefitinib Cell Line Tumor medicine Humans Epidermal growth factor receptor Phosphorylation neoplasms Molecular Biology Cell Proliferation EGFR inhibitors biology Chemistry Cell growth Cell Biology medicine.disease Molecular biology ErbB Receptors medicine.anatomical_structure Cell culture Quinazolines Cancer research biology.protein medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 358:226-232 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.04.124 |
| Popis: | We aimed to examine the expression of EGFR in neuroblastoma tissues and to investigate the antitumor activity of a selective EGFR-tyrosine kinase inhibitor, gefitinib, on neuroblastoma. The expression of EGFR was detected in each of two tumor tissues by immunohistochemistry and eight of 10 cell lines by Western blotting. Gefitinib inhibited EGFR-phosphorylation and in vitro cell growth (IC(50): approximately 1.2 microM), and a high concentration of gefitinib (20-30 microM) induced apoptosis in vitro. This is the first report that EGFR protein is expressed on the cell surface in neuroblastoma tissues and in cell lines. We also demonstrated an EGFR inhibitor induced apoptosis on neuroblastoma cells. Our results suggest the feasibility of targeting EGFR as a novel strategy against neuroblastoma. |
| Databáze: | OpenAIRE |
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