The Small GTPase Rab4A Interacts with the Central Region of Cytoplasmic Dynein Light Intermediate Chain-1
| Autor: | K A Fitzgerald, Alan G. Hendrick, Anna Bielli, Mary W. McCaffrey, Per-Ove Thörnqvist, Robert Finn |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cytoplasmic Dyneins
Molecular Sequence Data Dynein Biophysics HL-60 Cells Endosomes Spindle Apparatus Biology Microtubules Biochemistry Motor protein Jurkat Cells Mice chemistry.chemical_compound Microtubule Two-Hybrid System Techniques Animals Humans Amino Acid Sequence Cloning Molecular Molecular Biology Base Sequence Sequence Homology Amino Acid rab4 GTP-Binding Proteins Nocodazole Dyneins Cell Biology Cell biology Spindle apparatus chemistry Mutation Dynactin Rab Microtubule-Organizing Center HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 281:1141-1153 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.4468 |
| Popis: | Rab4 belongs to the Rab family of small GTPases involved in the regulation of intracellular transport, and has been localized to early endosomes. We have employed the yeast two-hybrid system to identify proteins that specifically interact with Rab4AQ67L, a GTPase-deficient mutant form of Rab4A. Screening a mouse embryo cDNA library identified a clone (M449) that interacted with Rab4A in a nucleotide-dependent fashion. Data base searches identified this clone as the mouse cytoplasmic dynein light intermediate chain-1 (LIC-1). Based on this finding, the full-length equivalent human cytoplasmic dynein LIC-1 was isolated by PCR. When Rab4A was overexpressed together with either M449 or dynein LIC-1 in HeLa cells, the proteins were found to colocalize in the perinuclear region. We characterize the localization of both overexpressed human dynein LIC-1 and the endogenous protein with respect to microtubules and show that it concentrates to the microtubule-organizing center and mitotic spindle. Additionally, GFPRab4A endosomes localize to microtubules and are redistributed by nocodazole treatment. This is the first described interaction between cytoplasmic dynein, a retrograde motor protein, and a Rab protein. |
| Databáze: | OpenAIRE |
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