Increased endothelial microparticles and oxidative stress at extreme altitude
| Autor: | Thomas Geiser, Alexander Benedikt Leichtle, Monika Stutz, Urs Hefti, Jacqueline Pichler Hefti, Andreas Huber, Tobias M. Merz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male CD31 medicine.medical_specialty Physiology medicine.medical_treatment Apoptosis 610 Medicine & health 030204 cardiovascular system & hematology Biology medicine.disease_cause Antioxidants Endothelial activation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Double-Blind Method Cell-Derived Microparticles Physiology (medical) Internal medicine medicine Humans Orthopedics and Sports Medicine Endothelial dysfunction Hypoxia Altitude Vitamin E Public Health Environmental and Occupational Health Vitamins General Medicine Phosphatidylserine Middle Aged Hypoxia (medical) medicine.disease Acetylcysteine Oxidative Stress Endocrinology chemistry Immunology Prostaglandins Female Endothelium Vascular medicine.symptom Biomarkers 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Pichler Hefti, Jacqueline Renée; Leichtle, Alexander Benedikt; Stutz, Monika; Hefti, Urs; Geiser, Thomas; Huber, Andreas R; Merz, Tobias Michael (2016). Increased endothelial microparticles and oxidative stress at extreme altitude. European journal of applied physiology, 116(4), pp. 739-748. Springer 10.1007/s00421-015-3309-3 |
| Popis: | PURPOSE Hypoxia and oxidative stress affect endothelial function. Endothelial microparticles (MP) are established measures of endothelial dysfunction and influence vascular reactivity. To evaluate the effects of hypoxia and antioxidant supplementation on endothelial MP profiles, a double-blind, placebo-controlled trial, during a high altitude expedition was performed. METHODS 29 participants were randomly assigned to a treatment group (n = 14), receiving vitamin E, C, A, and N-acetylcysteine daily, and a control group (n = 15), receiving placebo. Blood samples were obtained at 490 m (baseline), 3530, 4590, and 6210 m. A sensitive tandem mass spectrometry method was used to measure 8-iso-prostaglandin F2α and hydroxyoctadecadienoic acids as markers of oxidative stress. Assessment of MP profiles including endothelial activation markers (CD62+MP and CD144+MP) and cell apoptosis markers (phosphatidylserine+MP and CD31+MP) was performed using a standardized flow cytometry-based protocol. RESULTS 15 subjects reached all altitudes and were included in the final analysis. Oxidative stress increased significantly at altitude. No statistically significant changes were observed comparing baseline to altitude measurements of phosphatidylserine expressing MP (p = 0.1718) and CD31+MP (p = 0.1305). Compared to baseline measurements, a significant increase in CD62+MP (p = 0.0079) and of CD144+MP was detected (p = 0.0315) at high altitudes. No significant difference in any MP level or oxidative stress markers were found between the treatment and the control group. CONCLUSION Hypobaric hypoxia is associated with increased oxidative stress and induces a significant increase in CD62+ and CD144+MP, whereas phosphatidylserine+MP and CD31+MP remain unchanged. This indicates that endothelial activation rather than an apoptosis is the primary factor of hypoxia induced endothelial dysfunction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|