Immunogenicity of SARS inactivated vaccine in BALB/c mice
| Autor: | Chuiwen Qian, Chuan-hai Zhang, Min-Jie Meng, Shi-Sheng Liu, Huan-Yin Zheng, Jianglin Fan, Jiahai Lu, Xinjian Liu, Jiu-Xiang Li, Sheng Xiong, Wang Yifei, Zhuo-Yue Wan, Mei-Ying Zhang, Xin-Ge Yan |
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| Rok vydání: | 2004 |
| Předmět: |
Antigenicity
Immunology Antibodies Viral Severe Acute Respiratory Syndrome Article Neutralization BALB/c Mice Antibody Specificity Neutralization Tests IgG antibody Animals Immunology and Allergy skin and connective tissue diseases Antigens Viral Inactivated vaccine SARS Antiserum Mice Inbred BALB C biology Immune Sera Immunogenicity fungi Viral Vaccines biology.organism_classification Virology body regions Kinetics Titer Severe acute respiratory syndrome-related coronavirus Vaccines Inactivated Immunoglobulin G biology.protein Antibody |
| Zdroj: | Immunology Letters |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/j.imlet.2004.06.014 |
| Popis: | Severe acute respiratory syndrome (SARS) is a serious infectious threat to public health. To create a novel trial vaccine and evaluate its potency, we attempted to generate a SARS inactivated vaccine using SARS coronavirus (SARS-CoV) strain F69 treated with formaldehyde and mixed with Al(OH)3. Three doses of the vaccine were used to challenge three groups of BALB/c mice. We found that the mice exhibited specific IgM on day 4 and IgG on day 8. The peak titers of IgG were at day 47 in low-dose group (1:19,200) and high-dose group (1:38,400) whereas in middle-dose group (1:19,200), the peak was at day 40. On day 63, the IgG levels reached a plateau. Neutralization assay demonstrated that the antisera could protect Vero-E6 cells from SARS-CoV's infection. Analysis of the antibody specificity revealed that the mouse antisera contained a mixture of antibodies specifically against the structure proteins of SARS-CoV. Furthermore, the mouse antisera conferred higher amount of antibodies against protein N, polypeptide S4 and S2 than those of proteins M and 3CL. These findings suggest that the inactivated SARS-CoV could preserve its antigenicity and the inactivated vaccine can stimulate mice to produce high levels of antibodies with neutralization activity. Results also suggest that polypeptides originating from protein N or S might be a potential target for the generation of a recombinant SARS vaccine. |
| Databáze: | OpenAIRE |
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