SOX2 regulates homeostasis of taste bud cells and lingual epithelial cells in posterior tongue

Autor: Ichiro Matsumoto, Junpei Yamashita, Peihua Jiang, Makoto Ohmoto, Weiwei Lei, Junji Hirota
Rok vydání: 2020
Předmět:
0301 basic medicine
Sensory Receptors
Physiology
Cellular differentiation
Social Sciences
Gene Expression
Apoptosis
Epithelium
Mice
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Homeostasis
Psychology
Lingual papilla
In Situ Hybridization
education.field_of_study
Multidisciplinary
Cell Death
Stem Cells
Cell Differentiation
Taste Buds
Cell biology
medicine.anatomical_structure
Cell Processes
Taste
Medicine
Sensory Perception
Anatomy
Cellular Types
Stem cell
Research Article
Signal Transduction
Science
Population
Biology
03 medical and health sciences
Tongue
stomatognathic system
SOX2
Genetics
medicine
Animals
Progenitor cell
education
Mouth
SOXB1 Transcription Factors
Cognitive Psychology
Biology and Life Sciences
Epithelial Cells
Cell Biology
Embryonic stem cell
Biological Tissue
030104 developmental biology
Cognitive Science
Perception
Physiological Processes
Digestive System
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 10, p e0240848 (2020)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0240848
Popis: Taste bud cells arise from local epithelial stem cells in the oral cavity and are continuously replaced by newborn cells throughout an animal's life. However, little is known about the molecular and cellular mechanisms of taste cell turnover. Recently, it has been demonstrated that SOX2, a transcription factor expressed in epithelial stem/progenitor cells of the oral cavity, regulates turnover of anterior tongue epithelium including gustatory and non-gustatory papillae. Yet, the role of SOX2 in regulating taste cell turnover in the posterior tongue is unclear. Prompted by the fact that there are regional differences in the cellular and molecular composition of taste buds and stem/progenitor cells in the anterior and posterior portions of tongue, which are derived from distinct embryonic origins, we set out to determine the role of SOX2 in epithelial tissue homeostasis in the posterior tongue. Here we report the differential requirement of SOX2 in the stem/progenitor cells for the normal turnover of lingual epithelial cells in the posterior tongue. Sox2 deletion in the stem/progenitor cells neither induced active caspase 3-mediated apoptotic cell death nor altered stem/progenitor cell population in the posterior tongue. Nevertheless, morphology and molecular feature of non-gustatory epithelial cells were impaired in the circumvallate papilla but not in the filiform papillae. Remarkably, taste buds became thinner, collapsed, and undetectable over time. Lineage tracing of Sox2-deleted stem/progenitor cells demonstrated an almost complete lack of newly generated basal precursor cells in the taste buds, suggesting mechanistically that Sox2 is involved in determining stem/progenitor cells to differentiate to gustatory lineage cells. Together, these results demonstrate that SOX2 plays key roles in regulating epithelial tissue homeostasis in the posterior tongue, similar but not identical to its function in the anterior tongue.
Databáze: OpenAIRE
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