Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus
| Autor: | Rongjun Liu, Shujun Xu, Fang Xu, Wei Cui, Qinwen Wang, Wenhua Zhou, Jing Yang, Chuang Wang, Fan Lu, Zhao Li, Jinwei Zheng, Xiaowei Chen, Junfang Zhang, Junping Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Hippocampus Hippocampal formation NF-κB 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Fast Green FCF Neurotrophic factors Pharmacology (medical) TLR4 Neuroinflammation Original Research Pharmacology Chemistry Fast green FCF lipopolysaccharide lcsh:RM1-950 Interleukin Cell biology 030104 developmental biology lcsh:Therapeutics. Pharmacology 030220 oncology & carcinogenesis depression |
| Zdroj: | Frontiers in Pharmacology, Vol 10 (2019) Frontiers in Pharmacology |
| ISSN: | 1663-9812 |
| Popis: | Depression is a common neuropsychiatric disorder and new anti-depressive treatments are still in urgent demand. Fast Green FCF, a safe biocompatible color additive, has been suggested to mitigate chronic pain. However, Fast green FCF's effect on depression is unknown. We aimed to investigate Fast green FCF's effect on lipopolysaccharide (LPS)-induced depressive-like behavior and the underlying mechanisms. Pretreatment of Fast green FCF (100 mg/kg, i.p. daily for 7 days) alleviated depressive-like behavior in LPS-treated mice. Fast green FCF suppressed the LPS-induced microglial and astrocyte activation in the hippocampus. Fast green FCF decreased the mRNA and protein levels of Toll-like receptor 4 (TLR4) and Myeloid differentiation primary response 88 (Myd88) and suppressed the phosphorylation of nuclear factor-κB (NF-κB) in the hippocampus of LPS-treated mice. Fast green FCF also downregulated hippocampal tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, but did not alter the level of the brain-derived neurotrophic factor (BDNF) in the hippocampus of LPS-treated mice. The molecular docking simulation predicts that Fast green FCF may interact with TLR4 and interrupt the formation of the TLR4-MD2 complex. In conclusion, the anti-depressive action of Fast green FCF in LPS-treated mice may involve the suppression of neuroinflammation and the downregulation of TLR4/Myd88/NF-κB signal pathway in mouse hippocampus. Our findings indicate the potential of Fast green FCF for controlling depressive symptoms. |
| Databáze: | OpenAIRE |
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