Involvement of early embryonic miR-409-3p in the establishment of anxiety levels in female mice
| Autor: | Patrizia Stohn, Anne Harrington, Arturo Hernandez, Lucy Liaw, Larry A. Feig, David A. Dickson, Lorena Saavedra Rodriguez |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.drug_class Offspring Zygote Biology Anxiety Anxiolytic Article Epigenesis Genetic Andrology 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Human fertilization Developmental Neuroscience medicine Animals Behavior Animal Embryogenesis Embryo Embryo Mammalian Sperm Spermatozoa MicroRNAs 030104 developmental biology Female medicine.symptom 030217 neurology & neurosurgery Stress Psychological |
| Zdroj: | Dev Neurobiol |
| ISSN: | 1932-846X |
| Popis: | Small RNA molecules in early embryos, delivered from sperm to zygotes upon fertilization, are required for normal mouse embryonic development. Even modest changes in the levels of sperm-derived miRNAs appear to influence early embryos and subsequent development. For example, stress-associated behaviors develop in mice after injection into normal zygotes sets of sperm miRNAs elevated in stressed male mice. Here, we implicate early embryonic miR-409-3p in establishing anxiety levels in adult female, but not male mice. First, we found that exposure of male mice to chronic social instability stress, which leads to elevated anxiety in their female offspring across at least three generations through the paternal lineage, elevates sperm miR-409-3p levels not only in exposed males, but also in sperm of their F1 and F2 male offspring. Second, we observed that while injection of a mimic of miR-409-3p into zygotes from mating control males was incapable of mimicking this effect in offspring derived from them, injection of a specific inhibitor of this miRNA led to the opposite, anxiolytic effect in female, but not male, and offspring. These findings imply that baseline miR-409-3p activity in early female embryos is necessary for the expression of normal anxiety levels when they develop into adult females. In addition, elevated embryo miR-409-3p activity, possibly as a consequence of stress-induced elevation of its expression in sperm, may participate in, but may not be sufficient for, the induction of enhanced anxiety. |
| Databáze: | OpenAIRE |
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