Mercury-induced biochemical and proteomic changes in rice roots
| Autor: | Tsai Lien Huang, Thi Thuy Quynh Nguyeh, Yu Chywan Hsiung, Li Chiao Chia, Wen Chang Chi, Hao Jen Huang, Chung Yi Lin, Yun An Chen |
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| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Spectrometry Mass Electrospray Ionization Proteome Physiology Environmental pollution Plant Science medicine.disease_cause Plant Roots Superoxide dismutase chemistry.chemical_compound Ascorbate Peroxidases Plant Growth Regulators Gene Expression Regulation Plant Malondialdehyde Genetics medicine Homeostasis Electrophoresis Gel Two-Dimensional Plant Proteins chemistry.chemical_classification Reactive oxygen species biology Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase Oryza Mercury Glutathione Catalase APX Peroxidases chemistry Biochemistry biology.protein Oxidation-Reduction Oxidative stress Peroxidase |
| Zdroj: | Plant Physiology and Biochemistry. 55:23-32 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2012.03.008 |
| Popis: | Mercury (Hg) is a serious environmental pollution threats to the planet. Accumulation of Hg in plants disrupts many cellular-level functions and inhibits growth and development, but the mechanism is not fully understood. We investigated cellular, biochemical and proteomic changes in rice roots under Hg stress. Root growth rate was decreased and Hg, reactive oxygen species (ROS), and malondialdehyde (MDA) content and lipoxygenase activity were increased significantly with increasing Hg concentration in roots. We revealed a time-dependent alteration in total glutathione content and enzymatic activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) during Hg stress. 2-D electrophoresis revealed differential expression of 25 spots with Hg treatment of roots: 14 spots were upregulated and 11 spots downregulated. These differentially expressed proteins were identified by ESI-MS/MS to be involved in cellular functions including redox and hormone homeostasis, chaperone activity, metabolism, and transcription regulation. These results may provide new insights into the molecular basis of the Hg stress response in plants. |
| Databáze: | OpenAIRE |
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